Human Cystatin-B (CSTB/CST6/STFB) ELISA Kit CSB-E16835h from CUSABIO Technology LLC

Product Specs
ItemHuman Cystatin-B (CSTB/CST6/STFB) ELISA Kit
CompanyCUSABIO Technology LLC
Price $495.00Supplier Page View Company Product Page
Catalog NumberCSB-E16835h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namecystatin B
NCBI Gene AliasesCPI-B, CST6, EPM1, EPM1A, PME, STFB, ULD
Research AreaOthers
Assay Time1-5h
Detection Targetcystatin B (stefin B)
Volume50-100ul
Sensitivity0.233 ng/mL
Sample Typeserum, plasma, cell culture supernates, urine, saliva, tissue homogenates, cell lysates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range0.313 ng/mL-20 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CSTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CSTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CSTB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CSTB bound in the initial step. The color development is stopped and the intensity of the color is measured.

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