Human insulin-like 3 (INSL3) ELISA Kit CSB-E13909h from CUSABIO Technology LLC

Product Specs
ItemHuman insulin-like 3 (INSL3) ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-E13909h
Quantity96T
ApplicationsELISA
Detection Range0.312 ng/mL-20 ng/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
MethodELISA Kit
Sample Typeserum, plasma, tissue homogenates
Sensitivity0.078 ng/mL
Volume50-100ul
Detection Targetinsulin-like 3 (Leydig cell)
Assay Time1-5h
NCBI Full Gene Nameinsulin like 3
NCBI Gene AliasesRLF, RLNL, ley-I-L
Research AreaSignal Transduction

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INSL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INSL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INSL3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INSL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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