Human Interleukin-7,IL-7 ELISA Kit CSB-E14032h from CUSABIO Technology LLC

Product Specs
ItemHuman Interleukin-7,IL-7 ELISA Kit
CompanyCUSABIO Technology LLC
Price $468.00Supplier Page View Company Product Page
Catalog NumberCSB-E14032h
Quantity96T
Detection Range7.8 pg/mL-500 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, cell culture supernates, tissue homogenates
Sensitivity1.95 pg/mL
Volume50-100ul
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Nameinterleukin 7
NCBI Gene AliasesIL-7
Research AreaImmunology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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