Human Interleukin 10,IL-10 ELISA Kit CSB-E04593h from CUSABIO Technology LLC

Product Specs
ItemHuman Interleukin 10,IL-10 ELISA Kit
CompanyCUSABIO Technology LLC
Price $375.00Supplier Page View Company Product Page
Catalog NumberCSB-E04593h
Quantity96T
ApplicationsELISA
Detection Range31.25 pg/ml - 2000 pg/ml
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
MethodELISA Kit
Sample Typeserum, plasma, tissue homogenates
Sensitivity7.8 pg/ml
Volume50-100ul
Assay Time1-5h
NCBI Full Gene Nameinterleukin 10
NCBI Gene AliasesCSIF, GVHDS, IL-10, IL10A, TGIF
Research AreaImmunology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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CUSABIO Technology LLC

7707 Fannin St.

Ste 200-V126

Houston, TX 77054

United States

Phone: 301-363-4651

Email: support@cusabio.com

Company Profile

Website: www.cusabio.com

(1) METTL3-Driven m6A Epigenetic Remodeling of lncRNA-AU020206 Stabilizes SLC7A11 via YTHDC2 Attenuates Apoptosis and Ferroptosis in Cerebral Ischemia/Reperfusion InjuryBiomoleculesSeptember 24, 2025Hao Zhang, Yajin Guan, Meng Li, Yilin Wu, Xiaoou Sun(ELISA) Commercial ELISA kits specific for TNF-α (CSB-E04740h), IL-1β (CSB-E08053h), IL-6 (CSB-E04638h), and IL-10 (CSB-E04593h) were purchased from Wuhan Huamei Biotechnology Co., Ltd. (Wuhan, China). All reagents were prepared

(2) Serum from patients with oral squamous cell carcinoma remodels the tumor immune escape ecological niche by promoting regulatory T‑cell differentiation and T‑cell exhaustionOncology ReportsAugust 26, 2025Hongli Chen, Jiao Chen, Bomiao Cui, Die Lv, Wenwen Han, Yun Feng, Ping Zhangsupernatants were collected, and the concentrations of IL-4 (cat. no. CSB-E04633h), IL-10 (cat. no. CSB-E04593h) and TGF-β (cat. no. CSB-E04725h-IS) were determined using ELISA kits (Cusabio Technology, LLC) according

(3) METTL3 mediates m6A modification of lncRNA CRNDE to promote ATG10 expression and improve brain ischemia/reperfusion injury through YTHDC1Biology DirectOctober 16, 2024Zhengtao Yu, Ying Xia, Jiameng Li, Junwen Jiang, You Li, Youjun Li, Liu WangEnzyme-linked immunosorbent assay (ELISA) ELISA kits for TNF-α (CSB-E04740h), IL-1β (CSB-E08053h), IL-6 (CSB-E04638h), and IL-10 (CSB-E04593h) were obtained from Wuhan Huamei Biotechnology Co., Ltd. Reagents were prepared according to the manufacturer’s

(4) Increased opioid consumption in neoadjuvant immunotherapy plus chemotherapy for patients with non‐small‐cell lung cancer: A multicenter, prospective cohort studyCNS Neuroscience & TherapeuticsAugust 4, 2024Kaiyuan Wang, Jianxu Er, Yu Zhang, Chengcheng Song, Yang Yu, Ruifang Gao, Hong Xu, Xiaolin Dong, Limei Yuan, Qiangwei Liu, et al.levels of IL‐6, IL‐10, TNF‐α, and soluble PD‐L1 (sPD‐L1). The ELISA kit for IL‐6 (CSB‐E04638h), IL‐10 (CSB‐E04593h), TNF‐α (CSB‐E04740h), and sPD‐L1 (CSB‐E13644h) was purchased from CUSABIO. ELISA was conducted in accordance

(5) Chinese medicine PaBing-II protects human iPSC-derived dopaminergic neurons from oxidative stressFrontiers in ImmunologyAugust 2, 2024Shouhai Wu, Cuiping Rong, Ruishan Lin, Kaiyuan Ji, Tongxiang Lin, Weimin Chen, Wei Mao, Yang XuWuhan, China), interleukin 6 (IL-6) (CSB-E04638h, Cusabio, Wuhan, China), and interleukin 10 (IL-10) (CSB-E04593h, Cusabio, Wuhan, China). The following rat nigrostriatal region tissue ELISA kits were used: MCP-1 (CSB-E07429r,

(6) Lymphatic endothelial cell-mediated accumulation of CD177+Treg cells suppresses antitumor immunity in human esophageal squamous cell carcinomaOncoimmunologyMarch 22, 2024Min Ma, Liang Li, Shu-Han Yang, Chuan Huang, Weitao Zhuang, Shujie Huang, Xin Xia, Yong Tang, Zijun Li, Zhi-Bin Zhao, et al.PEPROTECH) at were added. After a stimulation period of 72 hours, the supernatants were collected for IL-10 (CSB-E04593h, CUSABIO) and IL-35 (CSB-E13126h, CUSABIO) ELISA assay. Cell lines and cell culture Human ESCC cell

(7) Lymphatic endothelial cell-mediated accumulation of CD177+Treg cells suppresses antitumor immunity in human esophageal squamous cell carcinomaOncoimmunologyMarch 20, 2024Min Ma, Liang Li, Shu-Han Yang, Chuan Huang, Weitao Zhuang, Shujie Huang, Xin Xia, Yong Tang, Zijun Li, Zhi-Bin Zhao, et al.PEPROTECH) at were added. After a stimulation period of 72 hours, the supernatants were collected for IL-10 (CSB-E04593h, CUSABIO) and IL-35 (CSB-E13126h, CUSABIO) ELISA assay. Cell lines and cell culture Human ESCC cell

(8) Circulating cytokine profile and modulation of regulatory T cells in chronic hepatitis B patients with type 2 diabetes mellitusBosnian Journal of Basic Medical SciencesFebruary 1, 2023Wang He, Shu Hao, Xiaohui Dong, Dandan Zhang, Zhen Jiacommercial enzyme-linked immunosorbent assay (ELISA) kits (CUSABIO, Wuhan, Hubei Province, China; Catalog# CSB-E04593h and CSB-E13126h) following manufacturer’s instructions. mRNA expression detection in Tregs mRNA expression

(9) Progesterone Enhances the Invasion of Trophoblast Cells by Activating PI3K/AKT Signaling Pathway to Prevent PreeclampsiaCell transplantationJanuary 1, 2023Liu H, Yu L, Ding Y, Peng M, Deng Y.pg/mL,CUSABIO (China) Rat IL-4,CSB-E04635r,15.6 pg/mL-1000 pg/mL,3.9 pg/mL,CUSABIO (China) Human IL-10,CSB-E04593h,31.25 pg/mL-2000 pg/mL,7.8 pg/mL,CUSABIO (China) Rat IL-10,CSB-E04595r,62.5 pg/mL-4000 pg/mL,15.6 pg/mL,CUSABIO

(10) Versatile-in-All-Trades: Multifunctional Boron-Doped Calcium-Deficient Hydroxyapatite Directs Immunomodulation and RegenerationACS Biomaterials Science & EngineeringJuly 11, 2022Ahmet Engin Pazarçeviren, Sema Akbaba, Zafer Evis, Ayşen Tezcanerkit (E4588, Biovision, USA). At the same time, aliquots were tested using a human IL-10 ELISA kit (CSB-E04593h, Cusabio, China). Afterward, media were discarded, cells were rinsed with PBS twice, and MTT reagent

(11) Luteolin activates M2 macrophages and suppresses M1 macrophages by upregulation of hsa_circ_0001326 in THP-1 derived macrophagesBioengineeredMarch 1, 2022Gong B, Zheng Y, Li J, Lei H, Liu K, Tang J, Peng Y.TNF-α (Cusabio, Wuhan, China; cat: CSB-E04740h), IL-6 (Cusabio, cat: CSB-E04638h), IL-10 (Cusabio, cat: CSB-E04593h), and IL-RA (Cusabio, cat: CSB-E04629h). Bioinformatics analysis To explore the potential mechanism

(12) Systemic low-grade inflammation and depressive symptomology at chronic phase of ischemic stroke: The chain mediating role of fibrinogen and neutrophil countsBrain, behavior, and immunityFebruary 1, 2022Sun W, Miao J, Song Y, Wang Y, Pan C, Li G, Zhao X, Lan Y, Qiu X, Zhu S, et al.

(13) Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male RatsEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2022Fan R, Dai F, Zhang C, Zhou L, Qiu X, Li Y.CSB-E04638h/CSB-E04639m), TNF-α ELISA Kit (Cusabio, CSB-E04740h/CSB-E04639m), IL-10 ELISA Kit (Cusabio, CSB-E04593h/CSB-E04594m), and IL-4 ELISA Kit (Cusabio, CSB-E04633h/CSB-E04634m) to detect the levels of NPY, T,

(14) Elevated DLL3 in stomach cancer by tumor-associated macrophages enhances cancer-cell proliferation and cytokine secretion of macrophagesGastroenterology reportJanuary 1, 2022Ye JB, Wen JJ, Wu DL, Hu BX, Luo MQ, Lin YQ, Ning YS, Li Y.(ELISA), including IL-1β (CSB-E08053h; CUSABIO; Wuhan, Hubei, China), IL-12 (CSB-E04599h; CUSABIO), IL-10 (CSB-E04593h; CUSABIO), and IL-33 (CSB-E13000h; CUSABIO). The results were quantified using a microplate reader (EPOCH2;

(15) The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart FailureMediators of InflammationOctober 27, 2021Zhenhua Wang, Zhaoling Cai, Markus W. Ferrari, Yilong Liu, Chengyi Li, Tianzhang Zhang, Guorong Lyulevel of IL-6 (CSB-E04638h, Cusabio biotech, China), IL-8 (CSB-E04641h, Cusabio biotech, China), IL-10 (CSB-E04593h, Cusabio biotech, China), and TNF-α (CSB-E04740h, Cusabio biotech, China) in the blood samples was determined

(16) Adipose-derived stem cells promote diabetic wound healing via the recruitment and differentiation of endothelial progenitor cells into endothelial cells mediated by the VEGF-PLCγ-ERK pathwayArchives of biochemistry and biophysicsOctober 15, 2020Chen L, Zheng Q, Liu Y, Li L, Chen X, Wang L, Wang L.

(17) Baicalin regulates Treg/Th17 cell imbalance by inhibiting autophagy in allergic rhinitisMolecular immunologySeptember 1, 2020Li J, Lin X, Liu X, Ma Z, Li Y.

(18) High lymphocyte‑to‑monocyte ratio is associated with low α‑fetoprotein expression in patients with hepatitis B virus‑associated hepatocellular carcinomaMolecular Medicine ReportsJuly 29, 2020Haixia Wang, Yu Xiang, Xinyu Li, Shuang Liu, Linxiu Liuand the expression levels of IL-10 were assessed using an ELISA kit (Human IL-10 ELISA kit; cat. no. CSB-E04593h; Cusabio Biotech Co., Ltd.) and MB-530 microplate reader to determine the cut-off value. Each group

(19) In major depression, increased kappa and mu opioid receptor levels are associated with immune activationActa neuropsychiatricaApril 1, 2020Al-Hakeim HK, Zeki Al-Fadhel S, Al-Dujaili AH, Maes M.

(20) MiR-18a-downregulated RORA inhibits the proliferation and tumorigenesis of glioma using the TNF-α-mediated NF-κB signaling pathwayEBioMedicineFebruary 1, 2020Jiang Y, Zhou J, Zhao J, Hou D, Zhang H, Li L, Zou D, Hu J, Zhang Y, Jing Z.

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Human Interleukin 10,IL-10 ELISA Kit from CUSABIO Technology LLC

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Human Interleukin 10,IL-10 ELISA Kit from CUSABIO Technology LLC

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