Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit CSB-E11172h from CUSABIO Technology LLC

Product Specs
ItemHuman A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-E11172h
Quantity96T
Detection Range7.8 pg/mL-500 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates
Sensitivity1.95 pg/mL
Volume50-100ul
Detection TargetADAM metallopeptidase domain 10
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameADAM metallopeptidase domain 10
NCBI Gene AliasesAD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz
Research AreaSignal Transduction

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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