Human CD226 antigen(CD226) ELISA Kit CSB-EL004901HU from CUSABIO Technology LLC

Product Specs
ItemHuman CD226 antigen(CD226) ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-EL004901HU
ApplicationsELISA
MethodELISA Kit
Research AreaImmunology
NCBI Gene AliasesDNAM-1, DNAM1, PTA1, TLiSA1
NCBI Full Gene NameCD226 molecule
Assay Time1-5h
Detection TargetCD226 molecule
Volume50-100ul
Sensitivity0.22 ng/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range0.9 ng/mL-60 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD226 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD226 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD226 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD226 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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