Human Soluble protein-100B,S-100B ELISA Kit CSB-E08065h from CUSABIO Technology LLC

Product Specs
ItemHuman Soluble protein-100B,S-100B ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-E08065h
Quantity96T
Detection Range78 pg/mL-5000 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
Sensitivity19.5 pg/mL
Volume50-100ul
Detection TargetS100 calcium binding protein B
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameS100 calcium binding protein B
NCBI Gene AliasesNEF, S100, S100-B, S100beta
Research AreaSignal Transduction

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100B bound in the initial step. The color development is stopped and the intensity of the color is measured.

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