Human coagulation factor Ⅸ,FⅨ ELISA Kit CSB-E08443h from CUSABIO Technology LLC

Product Specs
ItemHuman coagulation factor Ⅸ,FⅨ ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-E08443h
Quantity96T
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Volume50-100ul
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namecoagulation factor IX
NCBI Gene AliasesF9 p22, FIX, HEMB, P19, PTC, THPH8
Research AreaBlood Coagulation

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for F9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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