Human Interleukin-23 subunit alpha(IL-23A) ELISA Kit CSB-E17339h from CUSABIO Technology LLC

Product Specs
ItemHuman Interleukin-23 subunit alpha(IL-23A) ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-E17339h
Quantity96T
ApplicationsELISA
Detection Range62.5 pg/mL-4000 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
MethodELISA Kit
Sample Typeserum, plasma, cell culture supernates, tissue homogenates, cell lysates
Sensitivity15.6 pg/mL
Volume50-100ul
Detection Targetinterleukin 23, alpha subunit p19
Assay Time1-5h
NCBI Full Gene Nameinterleukin 23 subunit alpha
NCBI Gene AliasesIL-23, IL-23A, IL23P19, P19, SGRF
Research AreaImmunology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL23A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL23A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL23A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL23A bound in the initial step. The color development is stopped and the intensity of the color is measured.

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