Human Interleukin-31 receptor subunit alpha(IL31RA) ELISA Kit CSB-EL011654HU from CUSABIO Technology LLC

Product Specs
ItemHuman Interleukin-31 receptor subunit alpha(IL31RA) ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-EL011654HU
ApplicationsELISA
MethodELISA Kit
Research AreaImmunology
NCBI Gene AliasesCRL, CRL3, GLM-R, GLMR, GPL, IL-31RA, PLCA2, PRO21384, hGLM-R, zcytoR17
NCBI Full Gene Nameinterleukin 31 receptor A
Assay Time1-5h
Detection Targetinterleukin 31 receptor A
Volume50-100ul
Sensitivity1.56 pg/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range6.25 pg/mL-400 pg/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL31RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL31RA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL31RA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL31RA bound in the initial step. The color development is stopped and the intensity of the color is measured.

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