Human hepatocyte growth factor activator(HGFA) ELISA Kit CSB-E13160h from CUSABIO Technology LLC

Product Specs
ItemHuman hepatocyte growth factor activator(HGFA) ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-E13160h
Quantity96T
Detection Range78 pg/mL-5000 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates
Sensitivity19.5 pg/mL
Volume50-100ul
Detection TargetHGF activator
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameHGF activator
NCBI Gene AliasesHGFA
Research AreaCardiovascular

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HGFAC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGFAC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGFAC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGFAC bound in the initial step. The color development is stopped and the intensity of the color is measured.

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