Human heparin cofactor Ⅱ,HCⅡELISA Kit CSB-E09492h from CUSABIO Technology LLC

Product Specs
ItemHuman heparin cofactor Ⅱ,HCⅡELISA Kit
CompanyCUSABIO Technology LLC
Price $790.00Supplier Page View Company Product Page
Catalog NumberCSB-E09492h
Quantity96T
Detection Range1.25 pg/mL-80 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates
Sensitivity0.312 pg/mL
Volume50-100ul
Detection Targetserpin peptidase inhibitor, clade D (heparin cofactor), member 1
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Nameserpin family D member 1
NCBI Gene AliasesD22S673, HC2, HCF2, HCII, HLS2, LS2, THPH10
Research AreaOthers

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPIND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPIND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPIND1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPIND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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