Fig 1: Effects of the CIA development at both systemic and adipose tissue levels(A) Design of the study: control (n = 5) and CIA mice (n = 20).(B) Plasma levels of adipocytokines and insulin resistance in CIA mice (n = 20) compared with the control group (n = 5).(C) mRNA relative expression of genes involved insulin signal in gWAT of CIA (n = 20) and control group mice (n = 5).(D) mRNA relative expression of B cell markers in gWAT in CIA (n = 20) and control group mice (n = 5).(E) mRNA relative expression of markers of presence and polarization state of macrophages in gWAT of CIA (n = 20) and control group mice (n = 5).(F) B cell and macrophage presence by immunohistochemistry of gWAT in CIA (n = 10) and control group mice (n = 5).(G) Adipokine proteome profile in gWAT of CIA (n = 5) and control group mice (n = 5). CIA: collagen induced arthritis; gWAT: gonadal white adipose tissue; TNF-a: tumor necrosis factor alpha; MCP-1: monocyte chemotactic protein 1; CD: cluster differentiation; IRS: insulin receptor substrate; GLUT-4: glucose transporter type 4; adipoQ: adiponectin; HOMA-IR: homeostatic model assessment-insulin resistance; LCN-2: lipocalin-2; CRP: C-reactive protein; IGFBP: insulin like growth factor binding protein; DPP-4: dipeptidylpeptidase 4; AHSG: alpha 2-HS glycoprotein; PTX-3: pentraxin 3; SERPINE-1: serpin peptidase inhibitor, clade E member 1; LEP: leptin; TIMP-1: tissue inhibitor of metalloproteinase 1; LIF: leukemia inhibitory factor; DLK-1: delta like non-canonical notch ligand 1; ESM-1: endothelial cell specific molecule 1; FGF-21: fibroblast growth factor 21; IL-6: interleukin-6; MOK: MAPK/MAK/MRK overlapping kinase; H-E: hematoxylin-eosin. Significance was determined by t-test or Mann–Whitney rank-sum test for unpaired groups. *Significant differences vs control group, p < 0.05. Data are represented as mean ± SEM.