Human Dickkopf 3,DKK3 ELISA Kit CSB-E13607h from CUSABIO Technology LLC

Product Specs
ItemHuman Dickkopf 3,DKK3 ELISA Kit
CompanyCUSABIO Technology LLC
Price $468.00Supplier Page View Company Product Page
Catalog NumberCSB-E13607h
Quantity96T
Detection Range31.2 pg/mL-2000 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates
Sensitivity7.8 pg/mL
Volume50-100ul
Detection Targetdickkopf homolog 3 (Xenopus laevis)
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namedickkopf WNT signaling pathway inhibitor 3
NCBI Gene AliasesCRRL, REIC, RIG
Research AreaStem Cells

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DKK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DKK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKK3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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