Human Apolipoprotein C3,apo-C3ELISA Kit CSB-E13760h from CUSABIO Technology LLC

Product Specs
ItemHuman Apolipoprotein C3,apo-C3ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-E13760h
ApplicationsELISA
MethodELISA Kit
Research AreaMetabolism
NCBI Gene AliasesAPOCIII, Apo-C3, ApoC-3
NCBI Full Gene Nameapolipoprotein C3
Assay Time1-5h
Detection Targetapolipoprotein C-III
Volume50-100ul
Sensitivity3.9 ng/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range15.6 ng/mL-1000 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOC3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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