Human Macrophage Inflammatory Protein-3β,MIP-3β ELISA Kit CSB-E04670h from CUSABIO Technology LLC

Product Specs
ItemHuman Macrophage Inflammatory Protein-3β,MIP-3β ELISA Kit
CompanyCUSABIO Technology LLC
Price $468.00Supplier Page View Company Product Page
Catalog NumberCSB-E04670h
Quantity96T
ApplicationsELISA
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
MethodELISA Kit
Detection Range39 pg/ml-2500 pg/ml
Volume50-100ul
Detection Targetchemokine (C-C motif) ligand 19
Assay Time1-5h
Sample Typeserum, plasma, tissue homogenates
Sensitivity9.75 pg/ml
NCBI Full Gene NameC-C motif chemokine ligand 19
NCBI Gene AliasesCKb11, ELC, MIP-3b, MIP3B, SCYA19
Research AreaImmunology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL19 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL19 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Add to Compare List