Human Laminin subunit alpha-3(LAMA3) ELISA Kit CSB-EL012727HU from CUSABIO Technology LLC

Product Specs
ItemHuman Laminin subunit alpha-3(LAMA3) ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-EL012727HU
Quantity96T
ApplicationsELISA
Detection Range9.4 ng/mL-600 ng/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
MethodELISA Kit
Sample Typeserum, plasma, tissue homogenates
Sensitivity2.35 ng/mL
Volume50-100ul
Detection Targetlaminin, alpha 3
Assay Time1-5h
NCBI Full Gene Namelaminin subunit alpha 3
NCBI Gene AliasesBM600, E170, JEB2A, JEB2B, JEB2C, LAMNA, LOCS
Research AreaCardiovascular

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAMA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAMA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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