Human Interleukin-27 subunit beta(EBI3) ELISA Kit CSB-EL007360HU from CUSABIO Technology LLC

Product Specs
ItemHuman Interleukin-27 subunit beta(EBI3) ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-EL007360HU
Quantity96T
Detection Range15.6 pg/mL-1000 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates
Sensitivity3.9 pg/mL
Volume50-100ul
Detection TargetEpstein-Barr virus induced 3
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameEpstein-Barr virus induced 3
NCBI Gene AliasesIL-27B, IL27B, IL35B
Research AreaImmunology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EBI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EBI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EBI3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EBI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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