Human Serpin B5(SERPINB5) ELISA Kit CSB-EL021073HU from CUSABIO Technology LLC

Product Specs
ItemHuman Serpin B5(SERPINB5) ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-EL021073HU
Quantity96T
Detection Range0.156 ng/mL-10 ng/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates, cell lysates
Sensitivity0.039 ng/mL
Volume50-100ul
Detection Targetserpin peptidase inhibitor, clade B (ovalbumin), member 5
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Nameserpin family B member 5
NCBI Gene AliasesPI5, maspin
Research AreaOthers

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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