Human lipoprotein α,Lp-α ELISA Kit CSB-E08449h from CUSABIO Technology LLC

Product Specs
ItemHuman lipoprotein α,Lp-α ELISA Kit
CompanyCUSABIO Technology LLC
Price $495.00Supplier Page View Company Product Page
Catalog NumberCSB-E08449h
Research AreaCardiovascular
MethodELISA Kit
ApplicationsELISA
Assay Time1-5h
Detection Targetlipoprotein, Lp(a)
Volume50-100ul
Sensitivity53.106 ng/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range62.5 ng/mL-4000 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Lp-_, LPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Lp-_, LPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Lp-_, LPA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Lp-_, LPA bound in the initial step. The color development is stopped and the intensity of the color is measured.

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