Human Interleukin-34(IL34) ELISA Kit CSB-EL011657HU from CUSABIO Technology LLC

Product Specs
ItemHuman Interleukin-34(IL34) ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-EL011657HU
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Nameinterleukin 34
NCBI Gene AliasesC16orf77, IL-34
Research AreaImmunology
Assay Time1-5h
Detection Targetinterleukin 34
Volume50-100ul
Sensitivity5.86 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell culture supernates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range23.5 pg/mL-1500 pg/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL34 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL34 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL34 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL34 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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