Human cathepsin D,cath-D ELISA Kit CSB-E09221h from CUSABIO Technology LLC

Product Specs
ItemHuman cathepsin D,cath-D ELISA Kit
CompanyCUSABIO Technology LLC
Price $790.00Supplier Page View Company Product Page
Catalog NumberCSB-E09221h
Quantity96T
Detection Range83 pg/mL-2000 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates, ascitic fluid
Volume50-100ul
Detection Targetcathepsin D
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
Sensitivity33 pg/mL
NCBI Full Gene Namecathepsin D
NCBI Gene AliasesCLN10, CPSD, HEL-S-130P
Research AreaSignal Transduction

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTSD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSD bound in the initial step. The color development is stopped and the intensity of the color is measured.

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