Human VGF nerve growth factor inducible (VGF) ELISA Kit CSB-EL025847HU from CUSABIO Technology LLC

Product Specs
ItemHuman VGF nerve growth factor inducible (VGF) ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-EL025847HU
Quantity96T
Detection Range0.312 ng/mL-20 ng/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, cell culture supernates
Sensitivity0.078 ng/mL
Volume50-100ul
Detection TargetVGF nerve growth factor inducible
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameVGF nerve growth factor inducible
NCBI Gene AliasesSCG7, SgVII
Research AreaNeuroscience

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

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