Human 14-3-3 protein beta/alpha (YWHAB) ELISA Kit CSB-E12010h from CUSABIO Technology LLC

Product Specs
ItemHuman 14-3-3 protein beta/alpha (YWHAB) ELISA Kit
CompanyCUSABIO Technology LLC
Price $790.00Supplier Page View Company Product Page
Catalog NumberCSB-E12010h
Quantity96T
ApplicationsELISA
Detection Range6.25 ng/mL-400 ng/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
MethodELISA Kit
Sample Typeserum, plasma, tissue homogenates, cell lysates
Sensitivity1.56 ng/mL
Volume50-100ul
Detection Targettyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
Assay Time1-5h
NCBI Full Gene Nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
NCBI Gene AliasesGW128, HEL-S-1, HS1, KCIP-1, YWHAA
Research AreaOthers

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAB bound in the initial step. The color development is stopped and the intensity of the color is measured.

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CUSABIO Technology LLC

7707 Fannin St.

Ste 200-V126

Houston, TX 77054

United States

Phone: 301-363-4651

Email: support@cusabio.com

Company Profile

Website: www.cusabio.com

(1) Elevated levels of 14-3-3 proteins, serotonin, gamma enolase and pyruvate kinase identified in clinical samples from patients diagnosed with colorectal cancerClinica chimica acta; international journal of clinical chemistryFebruary 20, 2015Dowling P, Hughes DJ, Larkin AM, Meiller J, Henry M, Meleady P, Lynch V, Pardini B, Naccarati A, Levy M, et al.proteins were assayed using the quantitative sandwich enzyme immunoassay technique (CSB-E07961h and CSB-E12010h Cusabio Biotech, Wuhan, China). M2-PK levels were analysed using the ScheBo® • Tumor M2-PK™ EDTA Plasma

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Human 14-3-3 protein beta/alpha (YWHAB) ELISA Kit from CUSABIO Technology LLC

Specifications/Features

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Human 14-3-3 protein beta/alpha (YWHAB) ELISA Kit from CUSABIO Technology LLC

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