Human Myeloid cell surface antigen CD33(CD33) ELISA Kit CSB-EL004925HU from CUSABIO Technology LLC

Product Specs
ItemHuman Myeloid cell surface antigen CD33(CD33) ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-EL004925HU
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameCD33 molecule
NCBI Gene AliasesSIGLEC-3, SIGLEC3, p67
Research AreaImmunology
Assay Time1-5h
Detection TargetCD33 molecule
Volume50-100ul
Sensitivity31.25 pg/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range125 pg/mL-8000 pg/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD33 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD33 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD33 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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