Human Cytochrome P450 3A4(CYP3A4) ELISA Kit CSB-EL006439HU from CUSABIO Technology LLC

Product Specs
ItemHuman Cytochrome P450 3A4(CYP3A4) ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-EL006439HU
Quantity96T
Detection Range23.5 pg/mL-1500 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates, cell lysates
Sensitivity5.8 pg/mL
Volume50-100ul
Detection Targetcytochrome P450, family 3, subfamily A, polypeptide 4
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namecytochrome P450 family 3 subfamily A member 4
NCBI Gene AliasesCP33, CP34, CYP3A, CYP3A3, CYPIIIA3, CYPIIIA4, HLP, NF-25, P450C3, P450PCN1, VDDR3
Research AreaMetabolism

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP3A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP3A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP3A4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP3A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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