Human Thyroglobulin,TG ELISA Kit CSB-E04990h from CUSABIO Technology LLC

Product Specs
ItemHuman Thyroglobulin,TG ELISA Kit
CompanyCUSABIO Technology LLC
Price $520.00Supplier Page View Company Product Page
Catalog NumberCSB-E04990h
Quantity96T
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Volume50-100ul
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namethyroglobulin
NCBI Gene AliasesAITD3, TGN
Research AreaMetabolism
Sample Typeserum, plasma, tissue homogenates
Detection Range6.25 ng/ml- 400 ng/ml
Sensitivity1.56 ng/ml

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TG bound in the initial step. The color development is stopped and the intensity of the color is measured.

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