Human A Disintegrin And Metalloprotease 33,ADAM33 ELISA Kit CSB-E12702h from CUSABIO Technology LLC

Product Specs
ItemHuman A Disintegrin And Metalloprotease 33,ADAM33 ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-E12702h
Quantity96T
Detection Range31.25 pg/mL-2000 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, cell culture supernates, saliva, tissue homogenates
Sensitivity7.8 pg/mL
Volume50-100ul
Detection TargetADAM metallopeptidase domain 33
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameADAM metallopeptidase domain 33
NCBI Gene AliasesC20orf153, DJ964F7.1
Research AreaCell Biology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM33 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM33 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM33 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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