Human Interferon regulatory factor 7(IRF7) ELISA Kit CSB-EL011822HU from CUSABIO Technology LLC

Product Specs
ItemHuman Interferon regulatory factor 7(IRF7) ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-EL011822HU
Quantity96T
ApplicationsELISA
Detection Range23.4 pg/mL-1500 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
MethodELISA Kit
Sample Typeserum, plasma, tissue homogenates, cell lysates
Sensitivity5.8 pg/mL
Volume50-100ul
Detection Targetinterferon regulatory factor 7
Assay Time1-5h
NCBI Full Gene Nameinterferon regulatory factor 7
NCBI Gene AliasesIMD39, IRF-7, IRF-7H, IRF7A, IRF7B, IRF7C, IRF7H
Research AreaImmunology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IRF7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IRF7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IRF7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IRF7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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