Human Galectin-1(LGALS1) ELISA Kit CSB-EL012882HU from CUSABIO Technology LLC

Product Specs
ItemHuman Galectin-1(LGALS1) ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-EL012882HU
ApplicationsELISA
MethodELISA Kit
Research AreaCell Biology
NCBI Gene AliasesGAL1, GBP
NCBI Full Gene Namegalectin 1
Assay Time1-5h
Detection Targetlectin, galactoside-binding, soluble, 1
Volume50-100ul
Sensitivity0.78 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range3.12 ng/mL-200 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LGALS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LGALS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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