Human fibroblast growth factor 6 (FGF6) ELISA Kit CSB-E04549h from CUSABIO Technology LLC

Product Specs
ItemHuman fibroblast growth factor 6 (FGF6) ELISA Kit
CompanyCUSABIO Technology LLC
Price $468.00Supplier Page View Company Product Page
Catalog NumberCSB-E04549h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namefibroblast growth factor 6
NCBI Gene AliasesHBGF-6, HST2
Research AreaSignal Transduction
Assay Time1-5h
Detection Targetfibroblast growth factor 6
Volume50-100ul
Sensitivity1.95 pg/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range7.81 pg/mL-500 pg/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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