Human Complement component C8 beta chain(C8B) ELISA Kit CSB-EL004195HU from CUSABIO Technology LLC

Product Specs
ItemHuman Complement component C8 beta chain(C8B) ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-EL004195HU
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namecomplement C8 beta chain
NCBI Gene AliasesC82
Research AreaImmunology
Assay Time1-5h
Detection Targetcomplement component 8, beta polypeptide
Volume50-100ul
Sensitivity1.37 pg/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range2.74 pg/mL-2000 pg/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C8B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C8B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C8B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C8B bound in the initial step. The color development is stopped and the intensity of the color is measured.

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