Human Interleukin 4,IL-4 ELISA Kit CSB-E04633h from CUSABIO Technology LLC

Product Specs
ItemHuman Interleukin 4,IL-4 ELISA Kit
CompanyCUSABIO Technology LLC
Price $395.00Supplier Page View Company Product Page
Catalog NumberCSB-E04633h
ApplicationsELISA
MethodELISA Kit
Research AreaImmunology
NCBI Gene AliasesBCGF-1, BCGF1, BSF-1, BSF1, IL-4
NCBI Full Gene Nameinterleukin 4
Assay Time1-5h
Volume50-100ul
Sensitivity7.8 pg/ml
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range31.25 pg/ml - 2000 pg/ml
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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CUSABIO Technology LLC

7707 Fannin St.

Ste 200-V126

Houston, TX 77054

United States

Phone: 301-363-4651

Email: support@cusabio.com

Company Profile

Website: www.cusabio.com

(1) Exosomal miR-191 promotes colorectal cancer progression by inducing M2 macrophage polarization and inhibiting ferroptosisWJGODecember 15, 2025Qing-Yun Zhao, Shou-Jiang Weidehydrogenase (1:1000, ab9485; Abcam). Enzyme-linked immunosorbent assay The levels of interleukin (IL)-4 (CSB-E04633h; Cusabio, Wuhan, Hubei Province, China) and IL-10 (CSB-E04593h-IS; Cusabio) in cell supernatant or mouse

(2) Serum from patients with oral squamous cell carcinoma remodels the tumor immune escape ecological niche by promoting regulatory T‑cell differentiation and T‑cell exhaustionOncology ReportsAugust 26, 2025Hongli Chen, Jiao Chen, Bomiao Cui, Die Lv, Wenwen Han, Yun Feng, Ping ZhangCal-27 cells for 14 days. The supernatants were collected, and the concentrations of IL-4 (cat. no. CSB-E04633h), IL-10 (cat. no. CSB-E04593h) and TGF-β (cat. no. CSB-E04725h-IS) were determined using ELISA kits

(3) Progesterone Enhances the Invasion of Trophoblast Cells by Activating PI3K/AKT Signaling Pathway to Prevent PreeclampsiaCell transplantationJanuary 1, 2023Liu H, Yu L, Ding Y, Peng M, Deng Y.pg/mL,CUSABIO (China) Rat IL-1β,CSB-E08055r,0.156 ng/mL-10 ng/mL,0.039 ng/mL,CUSABIO (China) Human IL-4,CSB-E04633h,31.25 pg/mL-2000 pg/mL,7.8 pg/mL,CUSABIO (China) Rat IL-4,CSB-E04635r,15.6 pg/mL-1000 pg/mL,3.9 pg/mL,CUSABIO

(4) Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male RatsEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2022Fan R, Dai F, Zhang C, Zhou L, Qiu X, Li Y.CSB-E04740h/CSB-E04639m), IL-10 ELISA Kit (Cusabio, CSB-E04593h/CSB-E04594m), and IL-4 ELISA Kit (Cusabio, CSB-E04633h/CSB-E04634m) to detect the levels of NPY, T, IL-1β, IL-6, TNF-α, IL-10, and IL-4 in human and rat peripheral

(5) Kynurenine pathway in post-mortem prefrontal cortex and cerebellum in schizophrenia: relationship with monoamines and symptomatologyJournal of neuroinflammationSeptember 12, 2021Afia AB, Vila È, MacDowell KS, Ormazabal A, Leza JC, Haro JM, Artuch R, Ramos B, Garcia-Bueno B.min at 4°C. Cytokines and chemokine receptor levels IL-1β (Raybiotech, ELH-IL1b), IL-4 (Cusabio, CSB-E04633h) and IL-10 (Raybiotech, ELH-IL10) tissue levels were detected using a commercially ELISA-based kit following

(6) Lignosus rhinocerotis Cooke Ryvarden ameliorates airway inflammation, mucus hypersecretion and airway hyperresponsiveness in a murine model of asthmaPloS oneJanuary 1, 2021Johnathan M, Muhamad SA, Gan SH, Stanslas J, Mohd Fuad WE, Hussain FA, Wan Ahmad WAN, Nurul AA.

(7) Fabrication of facile polymeric nanocomposites based on chitosan-gr-P2-aminothiophenol for biomedical applicationsInternational journal of biological macromoleculesDecember 15, 2020Kamel EM, Ahmed OM, Abd El-Salam HM.

(8) TIMP3/TGF‑β1 axis regulates mechanical loading‑induced chondrocyte degeneration and angiogenesisMolecular medicine reportsOctober 1, 2020Zhao DL, Li HT, Liu SH.CSB-E14124h), interleukin (IL)-1β (cat. no. CSB-E08053h), IL-6 (cat. no. CSB-E04638h), IL-4 (cat. no. CSB-E04633h), IL-13 (cat. no. CSB-E04601h) and TGF-β1 (cat. no. CSB-E04725h), according to the manufacturers instructions.

(9) High molecular weight hyaluronic acid regulates P. gingivalis-induced inflammation and migration in human gingival fibroblasts via MAPK and NF-κB signaling pathwayArchives of oral biologyFebruary 1, 2019Chen M, Li L, Wang Z, Li P, Feng F, Zheng X.

(10) PKN2 in colon cancer cells inhibits M2 phenotype polarization of tumor-associated macrophages via regulating DUSP6-Erk1/2 pathwayMolecular cancerJanuary 24, 2018Cheng Y, Zhu Y, Xu J, Yang M, Chen P, Xu W, Zhao J, Geng L, Gong S.

(11) Elderly-onset hereditary pulmonary alveolar proteinosis and its cytokine profileBMC pulmonary medicineFebruary 17, 2017Ito M, Nakagome K, Ohta H, Akasaka K, Uchida Y, Hashimoto A, Shiono A, Takada T, Nagata M, Tohyama J, et al.

(12) CD32b expression is down-regulated on double-negative memory B cells in patients with Hashimoto's thyroiditisMolecular and cellular endocrinologyNovember 7, 2016Yalei Liu, Yan Gong, Chenxue Qu, Yang Zhang, Ran You, Nan Yu, Guizhi Lu, Youyuan Huang, Hong Zhang, Ying Gao, Yanming Gao, Xiaohui Guo

(13) Expressions of serum inflammatory cytokines and their relationship with cerebral edema in patients with acute basal ganglia hemorrhageEuropean review for medical and pharmacological sciencesJuly 1, 2016X-M Wang, Y-G Zhang, A-L Li, Z-H Long, D Wang, X-X Li, J-H Xia, S-Y Luo, Y-H Shan

(14) Interaction of coagulation factors and tumor-associated macrophages mediates migration and invasion of gastric cancerCancer scienceFebruary 1, 2011Ma YY, He XJ, Wang HJ, Xia YJ, Wang SL, Ye ZY, Tao HQ.

(15) Pro- and antiinflammatory cytokine balance in rheumatoid arthritisClinical and experimental rheumatologySeptember 1, 1995Miossec P.

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Human Interleukin 4,IL-4 ELISA Kit from CUSABIO Technology LLC

Specifications/Features

CUSABIO Technology LLC

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Human Interleukin 4,IL-4 ELISA Kit from CUSABIO Technology LLC

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