Human A Disintegrin And Metalloprotease 9,ADAM9 ELISA Kit CSB-E11904h from CUSABIO Technology LLC

Product Specs
ItemHuman A Disintegrin And Metalloprotease 9,ADAM9 ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-E11904h
Quantity96T
Detection Range62.5 pg/mL-4000 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates, cell culture supernates, ascitic fluid
Sensitivity15.6 pg/mL
Volume50-100ul
Detection TargetADAM metallopeptidase domain 9 (meltrin gamma)
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameADAM metallopeptidase domain 9
NCBI Gene AliasesCORD9, MCMP, MDC9, Mltng
Research AreaCancer

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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