Human Apolipoprotein C-IV(APOC4) ELISA Kit CSB-EL001934HU from CUSABIO Technology LLC

Product Specs
ItemHuman Apolipoprotein C-IV(APOC4) ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-EL001934HU
Quantity96T
Detection Range6.25 ng/mL-400 ng/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates
Sensitivity1.56 ng/mL
Volume50-100ul
Detection Targetapolipoprotein C-IV
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Nameapolipoprotein C4
NCBI Gene AliasesAPO-CIV, APOC-IV
Research AreaSignal Transduction

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOC4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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