Human Coagulation Factor XIII A1 Polypeptide(F13A1) ELISA Kit CSB-E14291h from CUSABIO Technology LLC

Product Specs
ItemHuman Coagulation Factor XIII A1 Polypeptide(F13A1) ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-E14291h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namecoagulation factor XIII A chain
NCBI Gene AliasesF13A
Research AreaBlood Coagulation
Assay Time1-5h
Detection Targetcoagulation factor XIII, A1 polypeptide
Volume50-100ul
Sensitivity0.156 ng/mL
Sample Typeserum, plasma
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range0.625 ng/mL-40 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for F13A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F13A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F13A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F13A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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