Glutathione Assay Kit (Fluorometric) ab65322 from Abcam

Product Specs
ItemGlutathione Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65322
Quantity100 Test
MethodFuncS
Sample TypeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
Assay Time2h 0m
Research AreaMetabolism

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Persistent PI3K–AKT signaling fortifies cellular defense against oxidative stress and ferroptosis through augmented mitochondrial fitnessCellular & Molecular Biology LettersMarch 14, 2026Xin Xie, Shanshan Wang, Wentao Zeng, Xiaohang Long, Daheng Zheng, Jianping Ye, Rachid Rezgui, Pu-Ste Liukit (ab235634), Luminescent ATP detection assay kit (ab113849), Glutathione assay kit (Fluorometric) (ab65322), phospho-AKT S473 antibody (ab81283), AKT antibody (ab8805), Anti-beta Actin antibody (ab8227), and

(2) Synergistic therapeutic effects of metformin and curcumin on polycystic ovary syndrome via regulation of insulin resistance and oxidative stress in a rat modelFront Endocrinol (Lausanne)February 16, 2026Lina Zheng, Baohua Wu, Junli Zhang, Yianduo Zhang, Zhiling Yang, Caifeng Deng, Chaolin Huang, Ling Lu(ab65354, Abcam) (39), Glutathione Peroxidase Assay Kit (ab102530, Abcam) (14), and Glutathione Assay Kit (ab65322, Abcam) (14), respectively. All procedures followed the manufacturer-provided protocols. 2.6 Ovarian

(3) PRMT1 drives oral squamous cell carcinoma progression by activating STAT3 and suppressing ferroptosis via GPX4Cell & BioscienceNovember 24, 2025Huawei Liu, Chuyan Shi, Bing Liu, Bo Wei, Yingying Wu, Peng Chenassay kit (ab118970; Abcam, Shanghai, China), and GSH levels were measured using the GSH assay kit (ab65322; Abcam, Shanghai, China), strictly following the protocols provided by the manufacturer. Absorbance

(4) Deficiency of 2‐Oxoglutarate Carrier (Slc25a11) Drives RPE Epithelial‐to‐Mesenchymal Transition and Exacerbates Subretinal Fibrosis in Neovascular Age‐Related Macular DegenerationAging CellOctober 28, 2025Mo Wang, Feng‐Juan Gao, Wenyi Tang, Boya Lei, Fangyuan Hu, Jun Ma, Srinivas R. Sadda, Ram Kannan, Parameswaran G. Sreekumar, Gezhi Xuprotein were loaded into a 96‐well plate, and GSH concentration was measured using a colorimetric assay (ab65322, Abcam) (Sreekumar et al. 2020). Total GSH levels were normalized to protein content and expressed as

(5) Alpinia galanga Essential Oil Mitigates Gentamicin-Induced Renal Injury: In silico and in vivo StudiesKidney Dis (Basel)September 2, 2025Saleh A. Alyami, Rand S. Alnaim, Renad K. Alaraifi, Dina M. Alnaim, Khaled S. Alnaim, Wassan A. Alnaim, Mohamed A. Albekery, Maged E. Mohamed, Nancy S. Youniskits were procured from Abcam Inc. Also, malondialdehyde (MDA, ab238537), glutathione content (GSH, ab65322) assay kits were purchased from Abcam Inc. Superoxide dismutase (SOD, Cat. No. 706002), glutathione

(6) Neuroprotective Potential of Broccoli Sprout Extract in Scopolamine-Induced Memory-Impaired MiceFoodsAugust 29, 2025Huijin Jeong, Hyukjoon Choi, Young-Seo Park(ab118970, Abcam Limited). Glutathione (GSH) levels were assessed using the Glutathione Detection Assay Kit (ab65322, Abcam Limited). 2.7. Brain Tissue Processing and Immunohistochemistry Brain tissue samples were fixed

(7) Curcumin inhibits ferroptosis through dessuccinylation of SIRT5-associated ACSL4 protein, and plays a chondroprotective role in osteoarthritisPLoS OneAugust 18, 2025Yong Xu, Yongxia Li, Lei Liu, Qingling Jing, Xiaojian Yeconcentrations were determined using iron assay kits (ab83366, Abcam, Cambridge, MA, USA), GSH assay kits (ab65322, Abcam), and MDA enzyme-linked immunosorbent assay (ELISA) kits (ab287797, Abcam), respectively, following

(8) Sanguinarine attenuates hypoxia/reoxygenation-triggered H9c2 cell injury through activation of the Nrf2/NLRP3 pathwayJournal of Clinical Biochemistry and NutritionApril 11, 2025Bo Qiu, Xin Li, Wenna Wangof malondialdehyde (MDA, ab118970; Abcam), superoxide dismutase (SOD, ab65354) and glutathione (GSH, ab65322) were adopted to examine the levels of MDA, SOD, and GSH in supernatant. Western blot The proteins

(9) Cistanche deserticola polysaccharide alleviates spinal cord injury in mice by inhibiting NLRP3 inflammasome activationNutr Res PractMarch 24, 2025Chengzhe Zhang, Juexin Shen, Hui Lian, Baohua Shi, Haitao Gong, Wanxin Liu, Yanting Shao, Zirui Ren, Dong Zhang× g for 10 min at 4°C. The level of MDA (ab118970; Abcam, Shanghai, China), SOD (ab65354), GSH-Px (ab65322), and CAT (ab83464) were measured in the supernatant using commercial kits according to the manufacturer’s

(10) Maslinic acid improves mitochondrial function and inhibits oxidative stress and autophagy in human gastric smooth muscle cellsOpen Life SciJanuary 27, 2025Xiaoying Zheng, Shuning Zhang, Qiaobin Chenperoxidase (GSH-Px) (oxidative stress) MDA (ab118970, Abcam, Shanghai, China), SOD (ab65354), and GSH-Px (ab65322) commercial kits were adopted to evaluate the levels of MDA, GSH, and SOD in the supernatant. 2.9 Statistical

(11) Baicalein inhibits NLRP3 inflammasome activation and mitigates placental inflammation and oxidative stress in gestational diabetes mellitusOpen Life SciDecember 31, 2024Jun Yao, Jiaying Pan, Qiaoying Jiang, Hui Wang, Yiqi Zhaostress markers, including malondialdehyde (MDA, ab118970, Abcam, Shanghai, China), glutathione (GSH, ab65322), myeloperoxidase (MPO, ab105136), and superoxide dismutase (SOD, ab65354), were measured using commercial

(12) The therapeutic effects of curcumin on polycystic ovary syndrome by upregulating PPAR-γ expression and reducing oxidative stress in a rat modelFront Endocrinol (Lausanne)November 20, 2024Wei Zhang, Cong Peng, Lei Xu, Yutai Zhao, Chaolin Huang, Ling LuChina), Glutathione Peroxidase Assay Kit (ab102530, Abcam, Shanghai, China), and Glutathione Assay Kit (ab65322, Abcam, Shanghai, China), respectively. All assays were performed according to the manufacturer’s instructions. 2.7

(13) Long noncoding RNA ADAMTS9-AS1 represses ferroptosis of endometrial stromal cells by regulating the miR-6516-5p/GPX4 axis in endometriosisScientific ReportsDecember 1, 2022Yiting Wan, Cancan Gu, Jueying Kong, Jin Sui, Ling Zuo, Yanhua Song, Jing ChenmiR-6516-5p or siGpx4. The glutathione (GSH) content in the ESCs was assayed using a glutathione assay kit (ab65322, Abcam). Lipid ROS level Lipid ROS levels were tested using a fluorescent-labeled oxidation-sensitive

(14) Deltamethrin-Evoked ER Stress Promotes Neuroinflammation in the Adult Mouse HippocampusCellsJune 18, 2022Hossain MM, Toltin AC, Gamba LM, Molina MA.lysis buffer and then the concentration of glutathione was measured using a glutathione assay kit (Cat #AB65322, Abcam, Cambridge, MA, USA), according to the manufacturer’s instructions. Briefly, samples and GSH

(15) Glabridin, a bioactive component of licorice, ameliorates diabetic nephropathy by regulating ferroptosis and the VEGF/Akt/ERK pathwaysMolecular medicine (Cambridge, Mass.)May 20, 2022Tan H, Chen J, Li Y, Li Y, Zhong Y, Li G, Liu L, Li Y.activity assay kit (#ab65354; Abcam), CAT assay kit (#CAT100; Sigma-Aldrich), fluorometric GSH assay kit (#ab65322; Abcam), lipid peroxidation (MDA) assay kit (#ab118970; Abcam), iron Assay kit (#ab83366; Abcam), and

(16) Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Ceacam1 GeneInternational journal of molecular sciencesApril 14, 2022Abu Helal R, Muturi HT, Lee AD, Li W, Ghadieh HE, Najjar SM.colorimetric Assay Kit, ab186032, Abcam, Cambridge, UK); Glutathione (GSH) (Glutathione detection assay kit, ab65322, Abcam); 8-isoprostane (8-isoprostane ELISA kit, ab175819, Abcam, Cambridge, UK); TNFα (SimpleStep ELISA

(17) Senkyunolide H inhibits activation of microglia and attenuates lipopolysaccharide‐mediated neuroinflammation and oxidative stress in BV2 microglia cells via regulating ERK and NF‐κB pathwayThe Kaohsiung Journal of Medical SciencesApril 1, 2022Jun Tan, Wei Li, Zhipeng Teng, Guang Wang, Yanyan Li, Yuan Zhang(ab100697; Abcam, Cambridge, MA, USA), IL‐6 (ab100772; Abcam), IL‐1β (ab100768; Abcam), glutathione (GSH) (ab65322; Abcam), catalase (CAT) (Catalase; RK02664; ABclonal, Wuhan, China), superoxide dismutase (SOD) (ab65354;

(18) HMGCR inhibition stabilizes the glycolytic enzyme PKM2 to support the growth of renal cell carcinomaPLOS BiologyApril 27, 2021Jiajun Huang, Xiaoyu Zhao, Xiang Li, Jiwei Peng, Weihao Yang, Shengli Mi(ELISA, R&D Systems, Minneapolis, MN, USA). GSH was determined by using Glutathione Assay Kit (Abcam, ab65322). Final VEGF and GSH levels were normalized to the total protein concentration. 2-NBDG uptake assay After

(19) Zoledronic acid promotes osteoclasts ferroptosis by inhibiting FBXO9-mediated p53 ubiquitination and degradationPeerJJanuary 1, 2021Qu X, Sun Z, Wang Y, Ong HS.absence of ZA (50 μM). GSH content in BMDMs and RAW264.7 cells was assayed using a Glutathione Assay Kit (ab65322; Abcam, Cambridge, England) according to the standard protocol. Briefly, cell supernatant, 5, 5′-dithio-bis

(20) Targeting the Cholecystokinin Receptor: A Novel Approach for Treatment and Prevention of Hepatocellular CancerCancer prevention research (Philadelphia, Pa.)January 1, 2021Gay MD, Safronenka A, Cao H, Liu FH, Malchiodi ZX, Tucker RD, Kroemer A, Shivapurkar N, Smith JP.carried out with ice cold perchloric acid (4 M), according to the Glutathione Assay Kit protocol (Abcam, ab65322). This fluorometric, dye-based assay provides a simple in vitro assay for detection of total glutathione

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Glutathione Assay Kit (Fluorometric) from Abcam

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Glutathione Assay Kit (Fluorometric) from Abcam

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