Description
Histamine is a heterocyclic primary amine derived from decarboxylation of the amino acid histidine. It is a mediator of inflammation closely associated with the initial phase of immediate hypersensitivity response (anaphylaxis). Histamine is synthesized by the enzyme histidine decarboxylase and is present in most cells, but typically stored in metachromatic granules of basophils and mast cells (granulocytes)(1). Neogen’s Histamine kit is designed for in vitro quantification of histamine in various fluids. Kit contents include: 1 x 96 well antibody-coated microplate, Standards (Ready-to-use), Enzyme Conjugate (HRP), K-Blue® Substrate, EIA Buffer, Wash Buffer (25X), PBS Sample Diluent, and Assay Protocol