Description
Cayman’s Thioredoxin Reductase Colorimetric Assay Kit provides a convenient method for detecting TrxR activity in tissue homogenates and cell lysates. It is based on the reduction of DTNB (5,5’-dithio-bis(2-dinitrobenzoic acid); Ellman’s reagent) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) which produces a yellow product that is measured at 405 nm.{14260,14261} The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity by DTNB reduction in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction (i.e., presence of glutathione).{14262} The difference between the two results is the DTNB reduction due to TrxR activity