Human Thrombospondin-2 Quantikine ELISA Kit DTSP20 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Thrombospondin-2 Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberDTSP20
Quantity1 Kit
ApplicationsELISA
Detection Range0.31 - 20 ng/mL
ReactivityHuman
Sample TypeCell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL), Human Milk (50 uL)
Sensitivity0.068 ng/mL
Detection TargetThrombospondin-2
Assay Time4.5 hours
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namethrombospondin 2
NCBI Gene AliasesTSP2

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Improving a Plasma Biomarker Panel for Early Detection of Pancreatic Ductal Adenocarcinoma with Aminopeptidase N (ANPEP) and Polymeric Immunoglobulin Receptor (PIGR)Clinical Cancer ResearchFebruary 17, 2026Brianna M. Krusen, Phyllis A. Gimotty, Greg Donahue, Jacob E. Till, Melinda Yin, Erin E. Carlson, William R. Bamlet, Erica L. Carpenter, Shounak Majumder, Ann L. Oberg, et al.#ab282302), human Aminopeptidase N/CD13 (R&D Systems Cat. #DY3815), human Thrombospondin-2 (R&D Systems Cat. #DTSP20), and human CA19-9 (Invitrogen™ Cat. #EHCA199) were used as described by the manufacturers’ instructions.

(2) Targeting fibroblast derived thrombospondin 2 disrupts an immune-exclusionary environment at the tumor front in colorectal cancerNature CommunicationsNovember 23, 2025Kosuke Iwane, Yuki Nakanishi, Yu Muta, Jiayu Chen, Kento Yasumura, Mayuki Omatsu, Naoki Aoyama, Munehiro Ikeda, Yoko Masui, Liyang Cai, et al.THBS2 in the serum was measured using the Human Thrombospondin-2 Quantikine ELISA Kit (R&D systems; DTSP20). Mouse serum samples were collected by standing still whole blood for 30 min followed by centrifugation.

(3) Circulating thrombospondin 2 as a predictor of hepatocellular carcinoma in hepatitis B patients undergoing nucleos(t)ide analog therapyScientific reportsOctober 26, 2024Okumura T, Kimura T, Ichikawa Y, Iwadare T, Wakabayashi SI, Kobayashi H, Yamashita Y, Uchida T, Pydi SP, Tanaka N, et al.levels were measured by a commercially available enzyme-linked immunoassay kit (QuantikineⓇ ELISA, #DTSP20, R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions using patient serum samples

(4) Thrombospondin 2 as a Predictive Biomarker for HCC in Hepatitis C Patients: A Longitudinal Study Following DAA TherapyJournal of Viral HepatitisOctober 15, 2024Takanobu Iwadare, Takefumi Kimura, Ayumi Sugiura, Taiki Okumura, Shun‐ichi Wakabayashi, Hiroyuki Kobayashi, Yuki Yamashita, Tomoo Yamazaki, Satoru Joshita, Naoki Tanaka, et al.Serum TSP2 concentrations were determined using enzyme‐linked immunosorbent assays (Quantikine ELISA, #DTSP20, R&D Systems, Minneapolis, MN) [7, 15, 16]. All serum samples were immediately stored at −30°C until

(5) Clinical phenotyping of plasma thrombospondin-2 reveals relationship to right ventricular structure and function in pulmonary hypertensionERJ Open ResearchMarch 13, 2023Anna M. Dittrich, Julia Mienert, Julian Pott, Lena Engels, Christoph Sinning, Jan K. Hennigs, Hans Klose, Lars Harbaumconcentration of plasma TSP2 levels were measured using a quantitative enzyme linked immunosorbent assay (DTSP20; R&D Systems, MN, United States). Based on duplicative measurements, mean coefficient of variation was

(6) Thrombospondin-2 as a Predictive Biomarker for Hepatocellular Carcinoma after Hepatitis C Virus Elimination by Direct-Acting AntiviralCancersJanuary 11, 2023Matsumae T, Kodama T, Tahata Y, Myojin Y, Doi A, Nishio A, Yamada R, Nozaki Y, Oshita M, Hiramatsu N, et al.at Osaka University. The serum TSP-2 level was examined with an ELISA kit for human TSP-2 (Catalog#DTSP20, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instruction. The absorbance was

(7) Circulating thrombospondin 2 levels reflect fibrosis severity and disease activity in HCV-infected patientsScientific reportsNovember 7, 2022Iwadare T, Kimura T, Tanaka N, Yamazaki T, Wakabayashi SI, Okumura T, Kobayashi H, Yamashita Y, Pydi SP, Nakajima T, et al.Serum TSP2 concentrations were determined using enzyme-linked immunosorbent assays (Quantikine® ELISA, #DTSP20, R&D Systems, Minneapolis, MN). Serum was obtained after overnight fasting on the day of liver biopsy

(8) The circulating proteomic signature of alcohol-associated liver diseaseJCI insightJuly 22, 2022Luther J, Vannier AG, Schaefer EA, Goodman RP.cohort. ELISAs from R&D Systems, Bio-Techne (DGD150 for GDF15, DST200 for IL1RL1, DFLRG0 for FSTL3, and DTSP20 for THBS2), were performed per the manufacturer’s instructions. Statistics. Data were compared by

(9) Thrombospondin-2 as a diagnostic biomarker for distal cholangiocarcinoma and pancreatic ductal adenocarcinomaClinical and Translational OncologyFebruary 1, 2022J. Byrling, K. S. Hilmersson, D. Ansari, R. Andersson, B. Anderssonquantitative sandwich enzyme-linked immunosorbent assay (ELISA) using a commercially available kit (DTSP20, R&D Systems, Minneapolis, MN, USA). The assay was performed according to the manufacturer’s instructions.

(10) THBS2/CA19–9 detecting pancreatic ductal adenocarcinoma at diagnosis underperforms in pre-diagnostic detection: Implications for biomarker advancementCancer Prevention ResearchFebruary 1, 2021Shirsa Udgata, Naomi Takenaka, William R. Bamlet, Ann L. Oberg, Stephanie S. Yee, Erica L. Carpenter, Daniel Herman, Jungsun Kim, Gloria M. Petersen, Kenneth S. Zaret1. Detecting THBS2 and CA19–9 levels in blood samples The THBS2 ELISA kit from Bio-Techne (R&D Systems DTSP20) was used as per the recommendations of the manufacturer except that to minimize lot-to-lot variation

(11) Pharmacologic Normalization of Pancreatic Cancer-Associated Fibroblast Secretome Impairs Prometastatic Cross-Talk With MacrophagesCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Samain R, Brunel A, Douché T, Fanjul M, Cassant-Sourdy S, Rochotte J, Cros J, Neuzillet C, Raffenne J, Duluc C, et al.kits (macrophage-CSF, DMC00B; connective tissue growth factor, DY9190-05; THSB2 (thrombospondin-2), DTSP20; all from R&D Systems, and biglycan ab245709; Abcam), according to the manufacturer’s instructions.

(12) Thrombospondin-2 is upregulated in patients with aortic dissection and enhances angiotensin II-induced smooth muscle cell apoptosisExperimental and therapeutic medicineDecember 1, 2020Qi L, Wu K, Shi S, Ji Q, Miao H, Bin Q.dilution with deionized water, 100 µl plasma solution was added to the plate and the TSP-2 (cat. no. DTSP20, R&D Systems, Inc.), TNF-α (cat. no. KHC3014C; Thermo Fisher Scientific, Inc.) and IL-6 (cat. no. EH2IL6;

(13) Gene Variants That Affect Levels of Circulating Tumor Markers Increase Identification of Patients With Pancreatic CancerClinical Gastroenterology and HepatologyMay 1, 2020Toshiya Abe, Chiho Koi, Shiro Kohi, Ki-Byung Song, Koji Tamura, Anne Macgregor-Das, Naoki Kitaoka, Miguel Chuidian, Madeline Ford, Mohamad Dbouk, et al.DRG International), CA125 (CA125, Quantikine; R&D Systems, Minneapolis, MN, USA), thrombospondin-2 (DTSP20, Quantikine; R&D Systems). Since CA125 levels are higher prior to menopause30, CA125 levels in women

(14) Combined Expression of Plasma Thrombospondin-2 and CA19-9 for Diagnosis of Pancreatic Cancer and Distal Cholangiocarcinoma: A Proteome ApproachThe oncologistApril 1, 2020Le Large TYS, Meijer LL, Paleckyte R, Boyd LNC, Kok B, Wurdinger T, Schelfhorst T, Piersma SR, Pham TV, van Grieken NCT, et al.in plasma samples with enzyme‐linked immunosorbent assay (ELISA). Human thromobospondin‐2 (THBS2, K#DTSP20, R&D Systems, Minneapolis, MN) and mucin 5B (MUC5B, #LS‐F22609, LSBio, Seattle, WA) were measured according

(15) A Blood-Based Multi Marker Assay Supports the Differential Diagnosis of Early-Stage Pancreatic CancerTheranosticsJanuary 1, 2019Berger AW, Schwerdel D, Reinacher-Schick A, Uhl W, Algül H, Friess H, Janssen KP, König A, Ghadimi M, Gallmeier E, et al.

(16) Thrombospondin-2 predicts response to treatment with intravenous immunoglobulin in children with Kawasaki diseaseBMJ paediatrics openJanuary 1, 2018Yang S, Song R, Li X, Zhang T, Fu J, Cui X.per the manufacturer’s instructions 2 (R&D Systems, USA, catalogue numbers: TSP-1: DTSP10, TSP-2: DTSP20). The blood samples for inflammatory indicators such as the count of peripheral blood white blood cell

(17) RBP4 and THBS2 are serum biomarkers for diagnosis of colorectal cancerOncotargetNovember 3, 2017Weiqiang Fei, Li Chen, Jiaxin Chen, Qinglan Shi, Lumin Zhang, Shuiping Liu, Lingfei Li, Lili Zheng, Xiaotong HuELISA which performed according to the procedure of R&D Systems (Catalog Number: RBP4 DRB400 and THBS2 DTSP20). The LOD of RBP4 Elisa kit DRB400 is 0.224 ng/mL and its detecting range is 1.6-100 ng/mL. The LOD

(18) Detection of early pancreatic ductal adenocarcinoma with thrombospondin-2 and CA19-9 blood markersScience translational medicineJuly 12, 2017Kim J, Bamlet WR, Oberg AL, Chaffee KG, Donahue G, Cao XJ, Chari S, Garcia BA, Petersen GM, Zaret KS.kits for human MMP2 (Millipore), human MMP10 (Ray Biotech), and human Thrombospondin-2 Quantikine (DTSP20, R&D systems) were used as described by the manufacturers’ instructions. Duplicate 5 ul plasma samples

(19) Circulating thrombospondin-2 reflects disease severity and predicts outcome of heart failure with reduced ejection fractionCirculation journal : official journal of the Japanese Circulation SocietyJanuary 1, 2014Hanatani S, Izumiya Y, Takashio S, Kimura Y, Araki S, Rokutanda T, Tsujita K, Yamamoto E, Tanaka T, Yamamuro M, et al.

(20) Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2The Journal of surgical researchSeptember 1, 2009Farrow B, Berger DH, Rowley D.

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Thrombospondin-2 ELISA

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Thrombospondin-2 ELISA

Thrombospondin-2 ELISA

Human Thrombospondin-2 Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Human Thrombospondin-2 Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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