Fig 1: Animal survival (A) and the neurological percentage structure (B) of experimental groups at 3 and 10 days following the intracerebral hemorrhage (according to the neurological testing data). BDNF, brain-derived neurotrophic factor; BU, BDNF + uPA; SO, sham-operated; uPA, urokinase-type plasminogen activator.
Fig 2: Brain-derived neurotrophic factor (BDNF), urokinase-type plasminogen activator (uPA) and their combination support the survival of SH-SY5Y neuroblastoma cells under glutamate-excitotoxic conditions (A) and stimulates neuritogenesis in SH-SY5Y cells (B). In (A), BDNF, uPA, and their combination (BU) significantly differ from the positive control (C+) group within the time range of 3–24 h: [*—H (3, 44) = 39.548, p = 0.001; **—H (3, 44) = 32.954, p = 0.001; ***—H (3, 44) = 24.681, p = 0.001; ****—H (3, 44) = 40.080, p = 0.001]. In (B), ×—H (3, 44) = 35800, p = 0.001; ××—H (3, 44) = 12.879, p = 0.005. Red dots on the diagram correspond to the result of individual measurements. All the comparisons were made versus the Control group value at the corresponding time point.
Fig 3: The results of immunohistochemical staining of brain slices 14 days following the intracerebral hemorrhage: (A)—CD68 and (B)—CD163. On the graphs, the dashed lines correspond to the CD68- or CD163-stained area in the brain slices of sham-operated (SO) animals and the red dots—to the result of individual measurements. The data are presented as the median (25%; 75%). *—H (3, 36) = 25.439, p = 0.001. **—H (3, 32) = 14.998, p = 0.002. BDNF, brain-derived neurotrophic factor; BU, BDNF + uPA; uPA, urokinase-type plasminogen activator.
Fig 4: The histological examination of brain slices. Hematoxylin-eosin staining reveals the site of damage (red dashed curved line). On the diagram: the dashed line corresponds to the area of the brain lesion in sham-operated (SO) animals and the red dots—to the result of individual measurements. The data are presented as the median (25%; 75%). *—H (3, 12) = 11.046, p = 0.011. BDNF, brain-derived neurotrophic factor; BU, BDNF + uPA; uPA, urokinase-type plasminogen activator.
Fig 5: The results of magnetic resonance imaging of brains 11 days following the intracerebral hemorrhage. On the images, the dashed red line corresponds to the volume of the brain lesion focus in sham-operated (SO) animals. Red dots on the diagram correspond to the result of individual measurements. The data are presented as the median (25%; 75%). *—H (3, 28) = 16.424, p = 0.001. BDNF, brain-derived neurotrophic factor; BU, BDNF + uPA; uPA, urokinase-type plasminogen activator.
Supplier Page from Abcam for Human uPA ELISA Kit (URK)