Human Pro-BDNF DuoSet ELISA DY3175 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Pro-BDNF DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY3175
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
Detection Range78.10 - 5000 pg/mL
ReactivityHuman
Detection TargetBDNF
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namebrain derived neurotrophic factor
NCBI Gene AliasesANON2, BULN2

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Machine learning helps predict early onset psychosis with serum protein biomarkers, neuropsychometry, and clinicodemographic dataScientific ReportsDecember 30, 2025Przemyslaw T. Zakowicz, Maksymilian A. Brzezicki, Joanna Pawlak, Maria Skibinska, Szymon Jurga, Aleksandra Lewandowska, Benedikt Vogel, Emily Ungermann, Barbara RemberkKits from R&D System (Minneapolis, MN, USA), specifically BDNF (cat. No.: DY248), proBDNF (cat. No.: DY3175), p75NTR (cat. No.: DY367), and S100B (cat. No.: DY1820-05). All assays were conducted according to

(2) Alterations in the plasma concentrations of BDNF, proBDNF, and MMP-9 in patients with schizophreniaEuropean Journal of Medical ResearchSeptember 26, 2025Yuhang Liang, Hongying Pan, Zhilan Yang, Chen Yu, Tiantian Jiang, Yinhang Li, Haiyan Yu, Min Qiu, Shaochuan ZhangKit (VAL 136, Bio-Techne, Bio-Techne China Co., Ltd., Shanghai, China), Human proBDNF DuoSet ELISA (DY3175, R&D Systems, Inc., Minneapolis, MN, USA), and Human MMP-9 DuoSet ELISA (DY911-05, R&D Systems, Inc.,

(3) Lactate infusion increases circulating pro-brain-derived neurotrophic factor levels in humansFrontiers in Cellular NeuroscienceSeptember 23, 2025Julia Röja, Nicolas Fiori Ameller, Jonathan Grip, William Apró, Marcus Mobergwere quantified with enzyme-linked immunosorbent assay (ELISA). The Human Pro-BDNF DuoSet ELISA kit (DY3175), in combination with the DuoSet Ancillary Reagent Kit 2 (DY008) from R&D Systems (Minneapolis, MN,

(4) Coping Styles and Defense Mechanisms in Healthy Young Adults—Correlations with tPA-BDNF PathwayBrain SciencesMay 26, 2025Julia Pilecka, Jedrzej Wojciechowski, Weronika Bargiel, Maria Terczynska, Przemyslaw Zakowicz, Dawid Bojarski, Karolina Wasicka-Przewozna, Maria Skibinskaplasma concentration quantification was performed using DuoSet ELISA kits (catalogue numbers DY248, DY3175, DY7449-05, DY9387-05, DY1470-05, DY911, respectively, R&D Systems, Minneapolis, MN, USA). ELISA was

(5) Monocyte-Platelet Aggregates Are Major Source of BDNF after Bacterial Stimulation of Human Peripheral Blood Immune CellsEuropean journal of immunologyApril 1, 2025Sarcletti F, Dijmarescu M, Eigenschink M, Wukowits N, Oehler B, Mayer T, Pell S, Tandecki A, Seki D, Spittler A, et al.

(6) Circulating Growth Factors and Cytokines Correlate with Temperament and Character Dimensions in Adolescents with Mood DisordersBrain sciencesJanuary 26, 2025Terczynska M, Bargiel W, Grabarczyk M, Kozlowski T, Zakowicz P, Bojarski D, Wasicka-Przewozna K, Kapelski P, Rajewska-Rager A, Skibinska M.

(7) Pro-Brain-Derived Neurotrophic Factor (BDNF), but Not Mature BDNF, Is Expressed in Human Skeletal Muscle: Implications for Exercise-Induced NeuroplasticityFunction (Oxf)May 2, 2024Sebastian Edman, Oscar Horwath, Thibaux Van der Stede, Sarah Joan Blackwood, Isabel Moberg, Henrik Strömlind, Fabian Nordström, Maria Ekblom, Abram Katz, William Apró, et al.Biotechnology (Dallas, TX, USA). Finally, a mouse monoclonal antibody supplied in the pro-BDNF ELISA kit (DY3175) from R&D Systems (Abingdon, UK) was reported to have 1.4% cross-reactivity with mBDNF was used. In

(8) The relationship between the brain-derived neurotrophic factor and neurocognitive response to physical exercise in individuals with schizophreniaPsychoneuroendocrinologyNovember 1, 2023Bang-Kittilsen G, Egeland J, Ueland T, Andersen E, Bigseth TT, Holmen TL, Mordal J, Holst R, Engh JA.

(9) Elevated Plasma Levels of Mature Brain-Derived Neurotrophic Factor in Major Depressive Disorder Patients with Higher Suicidal IdeationBrain sciencesAugust 21, 2023Li H, Zhao M, Jiang C, Zhao H, Wu C, Li Y, Zhang S, Xu P, Mou T, Xu Y, et al.Plasma proBDNF, TrkB, TNF-α, and IL-6 levels were estimated using a DuoSet human ELISA kit (proBDNF: DY3175; TrkB: DYC397; TNF-α: HSTA00E; IL-6: HS60DC—R&D Systems, Minneapolis, MN, USA) combined with a DuoSet

(10) The role of acute changes in mBDNF, cortisol and pro-BDNF in predicting cognitive performance in old ageScientific reportsJune 9, 2023Nilsson J, Ekblom M, Moberg M, Lövdén M.USA). Plasma levels of proBDNF (pg × ml−1) were quantified using the Human Pro-BDNF DuoSet ELISA kit (DY3175) combined with the DuoSet Ancillary Reagent Kit 2 (DY008) from R&D Systems (MN, USA). Assays were run

(11) α7 Nicotinic acetylcholine receptor potentiation downregulates chemotherapy-induced inflammatory overactivation by overlapping intracellular mechanismsThe international journal of biochemistry & cell biologyMay 1, 2023Marmouzi I, Myers S, Buck DJ, Davis RL, Arias HR.

(12) BDNF, proBDNF and IGF-1 serum levels in naïve and medicated subjects with autismScientific reportsAugust 12, 2022Robinson-Agramonte MLA, Michalski B, Vidal-Martinez B, Hernández LR, Santiesteban MW, Fahnestock M.and IGF-1 were measured40 using the Human BDNF DuoSet ELISA kit DY248, Human proBDNF DuoSet ELISA kit DY3175 and Human IGF-1 Quantikine ELISA kit DG100 (R&D Systems, Minneapolis, MN, US). The ancillary kit was

(13) BDNF and Pro-BDNF in Amyotrophic Lateral Sclerosis: A New Perspective for Biomarkers of NeurodegenerationBrain sciencesMay 9, 2022Riolo G, Ricci C, De Angelis N, Marzocchi C, Guerrera G, Borsellino G, Giannini F, Battistini S.

(14) The Effect of Physical Activity on Neurotrophin Concentrations and Cognitive Control in Patients With a Depressive EpisodeFrontiers in PsychiatryApril 25, 2022Anna JasiÅ„ska-MikoÅ‚ajczyk, Katarzyna Drews, Katarzyna Domaszewska, Grzegorz Kolasa, Marta Konofalska, Katarzyna Jowik, Maria SkibiÅ„ska, Filip Rybakowskianalyses were performed using DuoSet ELISA Development Kits (BDNF cat. no. DY248, proBDNF cat. no. DY3175, TrkB cat. no. DYC397-5, NGFR cat. no. DY367) from R&D Systems (Minneapolis, MN, USA) in accordance

(15) ProBDNF as an Indicator of Improvement among Women with Depressive EpisodesMetabolitesApril 16, 2022Zwolińska W, Skibinska M, Słopień A, Dmitrzak-Węglarz M.

(16) Clinical Correlates Identify ProBDNF and Thrombo-Inflammatory Markers as Key Predictors of Circulating p75NTR Extracellular Domain Levels in Older AdultsFrontiers in Aging NeuroscienceFebruary 21, 2022Samuel Fleury, Mireille E. Schnitzer, Lawrence Ledoux-Hutchinson, Imane Boukhatem, Jean-Christophe BÃ©langer, MÃ©lanie Welman, David Busseuil, Jean-Claude Tardif, Bianca Dâ€™Antono, Marie LordkipanidzÃ©proBDNF levels were assessed by enzyme-linked immunosorbent assay kits (R&D Systems, cat. DY367 and DY3175). Plates (96-well) were coated overnight with the capture antibody at room temperature according to

(17) Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative StressAntioxidants (Basel)January 10, 2022Ekaterina Novosadova et al.triplicate using Human/Mouse BDNF, Human Pro-BDNF and Human GDNF DuoSet ELISA kits (Cat. No: DY248, DY3175 and DY212, respectively; “R&D Systems”, Minneapolis, MN, USA), according to the manufacturer’s instructions. 3.

(18) Increased ratio of mature BDNF to precursor-BDNF in patients with major depressive disorder with severe anhedoniaJournal of psychiatric researchJuly 1, 2020Wu C, Lu J, Lu S, Huang M, Xu Y.

(19) Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNFFrontiers in immunologyJanuary 1, 2020Le Blanc J, Fleury S, Boukhatem I, Bélanger JC, Welman M, Lordkipanidzé M.and intraplatelet concentrations of BDNF and proBDNF were determined by ELISA (R&D System, DY248 and DY3175). Plasma and platelet lysate samples were centrifuged at 3,000 rpm for 5 min to remove cellular debris.

(20) N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structuresThe Journal of biological chemistryNovember 8, 2019Benicky J, Sanda M, Brnakova Kennedy Z, Goldman R.

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Pro BDNF ELISA

Human Pro-BDNF DuoSet ELISA

Human Pro-BDNF DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Human Pro-BDNF DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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