Human ULBP-2 DuoSet ELISA DY1298 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman ULBP-2 DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY1298
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
Detection Range62.50 - 4000 pg/mL
ReactivityHuman
Detection TargetULBP-2
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene NameUL16 binding protein 2
NCBI Gene AliasesALCAN-alpha, N2DL2, NKG2DL2, RAET1H, RAET1L

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Ectopic ULBP2 Is Associated with Decreased NKG2D Expression in CD8+ T Cells Under T Cell-Modulatory Conditions in a Murine Tumor ModelCellsJune 13, 2025Yasuhiko Teruya, Kosuke Yamaguchi, Kohei Yamane, Naomi Miyake, Yuji Nakayama, Takafumi Nonaka, Hiroki Chikumi, Akira Yamasakiconcentration of soluble ULBP2 in the culture supernatants was measured using the Human ULBP2 ELISA Kit (DY1298, R&D Systems). Cell surface expression of ULBP2 was analyzed by flow cytometry following staining with

(2) ULBP2 Promotes Tumor Progression by Suppressing NKG2D-Mediated Anti-Tumor ImmunityInternational Journal of Molecular SciencesMarch 24, 2025Kohei Yamane, Kosuke Yamaguchi, Yasuhiko Teruya, Naomi Miyake, Yuji Nakayama, Takafumi Nonaka, Hiroki Chikumi, Akira Yamasakiconcentration of soluble ULBP2 in the culture supernatants was measured using the Human ULBP2 ELISA Kit (DY1298, R&D Systems). ULBP2 expression on the cell surface was analyzed by performing flow cytometry following

(3) 23ME-01473, an Fc Effector-Enhanced Anti-ULBP6/2/5 Antibody, Restores NK Cell-Mediated Antitumor Immunity through NKG2D and FcγRIIIa ActivationCancer Res CommunMarch 1, 2025Benjamin JS, Jarret A, Bharill S, Fontanillas P, 23andMe Research Team, Yadav S, Sen D, Ayupova D, Kellar D, Tilk S, et al.also harvested for soluble ULBP6/2/5 quantification using a ULBP-2 DuoSet ELISA (R&D Systems, Cat# DY1298). Briefly, 96-well plates were coated with the capture antibody overnight at room temperature. The next

(4) HCMV Variants Expressing ULBP2 Enhance the Function of Human NK Cells via its Receptor NKG2DEuropean journal of immunologyFebruary 1, 2025Meyer G, Siemes AR, Kühne JF, Bevzenko I, Baszczok V, Keil J, Beushausen K, Wagner K, Steinbrück L, Messerle M, et al.(sULBP2) in cell culture supernatants were quantified with Human ULBP‐2 DuoSet ELISA (R&D Systems; cat. DY1298) according to the manufacturer's instructions. Supernatants taken from uninfected and TB40(R)‐infected

(5) Clearing soluble MIC reverses the impaired function of natural killer cells from patients with multiple myelomaJournal for ImmunoTherapy of CancerJanuary 8, 2024Sojeong Kim, Haerim Chung, Jeong-Eun Kwak, Yu Ri Kim, Chung Hyun Park, Yeonhee Kim, June-Won Cheong, Jennifer Wu, Eui-Cheol Shin, Hyunsoo Cho, et al.ELISA (R&D Systems, Minnesota, USA) for human MICA (DY1300), MICB (DY1599), ULBP1 (DY1380), ULPB2 (DY1298) and ULBP3 (DY1517-05), using the procedures recommended by the manufacturers. Briefly, 96-well microplate

(6) Cross-Dressing of Multiple Myeloma Cells Mediated by Extracellular Vesicles Conveying MIC and ULBP Ligands Promotes NK Cell KillingInternational journal of molecular sciencesMay 30, 2023Vulpis E, Loconte L, Cassone C, Antonangeli F, Caracciolo G, Masuelli L, Fazio F, Petrucci MT, Fionda C, Soriani A, et al.

(7) SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2DCell reportsDecember 27, 2022Lee MJ, Leong MW, Rustagi A, Beck A, Zeng L, Holmes S, Qi LS, Blish CA.Human MICA DuoSet ELISA (R&D Systems, Cat. DY1800) and Human ULBP-2 DuoSet ELISA (R&D Systems, Cat. DY1298) kits according to the manufacturer’s instructions. 1% Triton-X 100 (Sigma-Aldrich, Cat. T9284-100ML)

(8) Histone Deacetylase Inhibitor Modulates NKG2D Receptor Expression and Memory Phenotype of Human Gamma/Delta T Cells Upon Interaction With Tumor CellsFrontiers in immunologyJanuary 1, 2019Bhat J, Dubin S, Dananberg A, Quabius ES, Fritsch J, Dowds CM, Saxena A, Chitadze G, Lettau M, Kabelitz D.coating with antibodies to quantitate soluble MICA, MICB, ULBP-1, ULBP-2 (#DY1300, #DY1599, #DY1380, #DY1298, respectively; all from R&D Systems) by ELISA according to manufacturer's protocol. Co-culture Assays For

(9) A disease-linked ULBP6 polymorphism inhibits NKG2D-mediated target cell killing by enhancing the stability of NKG2D ligand bindingScience SignalingMay 30, 2017Jianmin Zuo et al.CHO-ULBP0601, and CHO-ULBP0602 cells was harvested and analyzed with a Human ULBP-2 DuoSet ELISA kit (R&D system DY1298). The concentration of ULBP6 was calibrated according to the recommendation from the product datasheet:

(10) NKG2D- and T-cell receptor-dependent lysis of malignant glioma cell lines by human γδ T cells: Modulation by temozolomide and A disintegrin and metalloproteases 10 and 17 inhibitorsOncoimmunologyApril 1, 2016Chitadze G, Lettau M, Luecke S, Wang T, Janssen O, Fürst D, Mytilineos J, Wesch D, Oberg HH, Held-Feindt J, et al.supernatants were subjected to ELISA. The amounts of sMICB (#DY1599), sULBP1 (#DY1380) and sULBP2 (#DY1298) were determined with the respective sandwich ELISA kits (R&D Systems) following the manufacturer's

(11) Expression and prognostic significance of unique ULBPs in pancreatic cancerOncoTargets and therapyJanuary 1, 2016Chen J, Zhu XX, Xu H, Fang HZ, Zhao JQ.

(12) Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicityEuropean journal of immunologyAugust 1, 2015Cox ST, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal JA, Saudemont A.

(13) Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroidsBMC cancerMay 3, 2015Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer EH, Cerwenka A, Steinle A, Koehl U, Koch J.Germany). Soluble MICB, ULBP1 and ULBP2 levels were quantified by DuoSet ELISA kits (DY1599, DY1380, DY1298, R&D Systems, USA), following the manufacturer’s instructions. Soluble MICA was analyzed as described

(14) Soluble ligands for the NKG2D receptor are released during endometriosis and correlate with disease severityPloS oneJanuary 1, 2015González-Foruria I, Santulli P, Chouzenoux S, Carmona F, Batteux F, Chapron C.

(15) Natural killer cell-mediated shedding of ULBP2PloS oneJanuary 1, 2014Wang R, Sun PD.

(16) Combined proteome and transcriptome analyses for the discovery of urinary biomarkers for urothelial carcinomaBritish Journal of CancerMay 14, 2013N J Shimwell, R T Bryan, W Wei, N D James, K K Cheng, M P Zeegers, P J Johnson, A Martin, D G Wardwere measured in urine and serum sample using commercially available ELISA kits (DY258, DY1048 and DY1298, R&D systems, Abingdon, UK) according to the manufacturer's instructions. Standards and samples were

(17) Soluble ligands for NK cell receptors promote evasion of chronic lymphocytic leukemia cells from NK cell anti-tumor activityBloodMay 2, 2013Katrin S. Reiners, Daniela Topolar, Alexander Henke, Venkateswara R. Simhadri, JÃ¶rg Kessler, Maike Sauer, Martina Bessler, Hinrich P. Hansen, Samir Tawadros, Marco Herling, et al.DY1300), DuoSet Human MICB (Cat. DY1599), DuoSet Human MIF (Cat. DY289) and DuoSet Human ULBP2 (Cat. DY1298). In a pilot experiment, standards were diluted either in PBS or human plasma samples. Interestingly,

(18) Rescue of impaired NK cell activity in hodgkin lymphoma with bispecific antibodies in vitro and in patientsMolecular therapy : the journal of the American Society of Gene TherapyApril 1, 2013Reiners KS, Kessler J, Sauer M, Rothe A, Hansen HP, Reusch U, Hucke C, Köhl U, Dürkop H, Engert A, et al.

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ULBP-2 ELISA

Human ULBP-2 DuoSet ELISA

Human ULBP-2 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Human ULBP-2 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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