Description
Introduction: Macrophage Colony Stimulating Fcator (M-CSF), also known as CSF-1, can be either expressed on the cell surface as a membrane-spanning 68-86 kDa chondroitin or secreted as an 80-100 kDa glycoprotein or 130-160 kDa chondroitin sulfate-containing proteoglycan. The biologically active forms of M-CSF are dimeric. The following tissues are known producers of M-CSF: submaxillary gland, lung, spleen, kidney, lymph nodes, brain, liver, testis, ovary, and some human tumors. M-CSF can be synthesized in most cell types including fibroblasts, endothelial cells, bone marrow stromal cells, osteoblasts, thymic epithelial cells, keratinocytes, astrocytes, myoblasts, mesothelial cells, liver parenchymal cells, thyrocytes, and adipocytes. The primary biological activities of M-CSF are related to the survival, proliferation, and differentiation of mononuclear phagocytes. Many conditions are accompanied by elevated M-CSF levels, such as pregnancy, neoplastic disorders of hematopoietic and reproductive systems, pre-edampsia, chemotherapy with and without autologous bone marrow transplantation, infection, liver disease, hepatic injury, hemophagocytic syndrome, thalassemia, amyloidosis, ischemic heart disease, ovarian cancer, endometrial cancer, breast cancer, and amyloidosis.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to M-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for M-CSF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain M-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of M-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve