3-Nitrotyrosine ELISA Kit ab116691 from Abcam

Product Specs
Item3-Nitrotyrosine ELISA Kit
CompanyAbcam
Price $495.00Supplier Page View Company Product Page
Catalog Numberab116691
Quantity1 x 96 Tests
MethodsELISA
ApplicationssELISA, ELISA
Detection Range8 - 1000 ng/ml
SensitivityEqual to 8 ng/mL

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Prenatal bisphenol A exposure reprograms SF1-lactylation pathways to promote endometriosis susceptibilityiScienceMay 15, 2026Xiaohan Liu, Yanxia Fu, Liuxuan Huang, Donghan Li, Paul Yao, Liqin Zeng485/530nm and expressed in arbitrary units. 3-nitrotyrosine levels were assessed with an ELISA kit (Abcam, #ab116691). γH2AX was quantified via western blotting of nuclear fractions, with H2AX as the loading control.47 Mitochondrial

(2) Fenofibrate attenuates the adverse effects of radiation on endothelial cells through modulation of ROS-NO signalling and inflammationRedox BiologyFebruary 1, 2026Omar Elsaeed, Christine von Toerne, Prabal Subedi, Louis Wilhelm, Lukas Duchrow, Ali Bashiri Dezfouli, Gabriele Multhoff, Simone Moertl, Omid Azimzadehin cell lysates were measured as a marker for nitrosative stress and oxidative damage (Abcam, USA, ab116691). The results for both assays were normalized to 100 μg protein input per sample. 5.16 SOD and NOX

(3) Hevin Promotes Aging‐Related Cardiac Dysfunction via Facilitating Cardiac Inflammation in Male MiceAging CellFebruary 1, 2026Shi‐Yu Huang, Yu‐Jie Chen, Yu‐Xin Hu, Jia‐Chen Liu, Min Hupurchased from Cell Signaling Technology (Danvers, MA, USA). 3‐Nitrotyrosine (3‐NT) ELISA Assay Kit (#ab116691) was purchased from Abcam (Cambridge, UK). AAV9 vectors carrying the short hairpin RNA against Hevin

(4) Peroxynitrite regulates ER stress-mediated Ca2+ flux to mitochondria characterizing cardiac microvascular ischemia–reperfusion injury associated with hyperhomocysteinemiaJournal of Translational MedicineNovember 10, 2025Haipeng Liu, Siyang Yu, Shansong Gao, Xiaoming Liu, Chengpeng Qiu, Ning Wang, Xinyu Tan, Kaiyang Zhao, Qiuyu He, Wan Zhangsoftware. 3-Nitrotyrosine detection 3-Nitrotyrosine (3-NT) was determined using the 3-Nitrotyrosine ELISA Kit (Abcam, USA, ab116691) according to the manufacturers’ instructions. Cells were seeded in 6-well plates at a density of 800

(5) Air pollution modulates brown adipose tissue function through epigenetic regulation by HDAC9 and KDM2BJCI InsightSeptember 23, 2025Rengasamy Palanivel, Jean-Eudes Dazard, Bongsoo Park, Sarah Costantino, Skanda T. Moorthy, Armando Vergara-Martel, Elaine Ann Cara, Jonnelle Edwards-Glenn, Shyam Biswal, Lung Chi Chen, et al.peroxynitrite, were measured in serum-treated mature brown adipocytes by a commercially available ELISA kit (ab116691, Abcam). Oxygen consumption rate and lactate level. The oxygen consumption rate (OCR) and lactate

(6) Targeting the Epigenetic Remodeler GCN5 Prevents Vascular Oxidative Stress and Endothelial Dysfunction in Obesity: Insights in Patients with Cardiometabolic DiseaseHigh Blood Pressure & Cardiovascular PreventionSeptember 8, 2025Sarah Costantino, Shafeeq Mohammed, Alessandro Mengozzi, Emiliano Duranti, Valentina Delfine, Martin A. Geiger, Nazha Hamdani, Stefano Taddei, Stefano Masi, Agostino Virdis, et al.of oxidative damage mediated by peroxynitrite, were measured by a commercially available ELISA kit (ab116691, Abcam) in vascular homogenates and cell lysates. Assessment of nitric oxide levels The levels of

(7) Inhibition of AMPKα Pathway by Podocyte GOLM1 Exacerbates Diabetic Nephrology in MiceAdvanced ScienceJuly 13, 2025Peng Xu, Kaiqiang Li, Huasong Liu, Aibo Xu, Zhewei Zhang, Yimin Yang, Xiaobo Lai, Ke Hao, Kui Fang, Zeyu Lai, et al.(MDA), 3‐Nitrotyrosine (3‐NT), and 8‐hydroxy 2 deoxyguanosine (8‐OHdG), commercial kits (#ab118970, #ab116691, #ab201734, Abcam, Cambridge, UK) were used. To measure NF‐κB activity, nuclear extracts were prepared

(8) Current understanding of eryptosis: mechanisms, physiological functions, role in disease, pharmacological applications, and nomenclature recommendationsCell Death DisJuly 1, 2025Anton Tkachenko, Mohammad A. Alfhili, Jawaher Alsughayyir, Alessandro Attanzio, Abdulla Al Mamun Bhuyan, Bożena Bukowska, Antonio Cilla, Martha A. Quintanar-Escorza, Michael Föller, Ondrej Havranek, et al.colorimetric kits.vi.Protein damage – tryptophan fluorescence (decrease in OD).vii.3-nitrotyrosine – Abcam #ab116691.viii.Antioxidant enzymes (CAT, SOD) – colorimetric/ELISA/FCM kits.ix.Glutathione system (GSH, GSSG,

(9) FNDC4 Prevents Aging-Related Cardiac Dysfunction: By Restoring AMPKα/PPARα-Dependent Mitochondrial FunctionJACC: Basic to Translational ScienceJuly 1, 2025Xin Zhang, Wen-Sheng Dong, Kang Li, Yun-Jia Ye, Can HuComplex IV Rodent Enzyme Activity Microplate Assay Kit (#ab109911), 3-Nitrotyrosine (3-NT) ELISA Assay Kit (#ab116691) and cAMP Assay Kit (#ab65355) were purchased from Abcam. Triglyceride (TG) Assay Kit (#A110), Malondialdehyde

(10) Dysregulated inflammation, oxidative stress, and protein quality control in diabetic HFpEF: unraveling mechanisms and therapeutic targetsCardiovascular diabetologyMay 14, 2025Delalat S, Sultana I, Osman H, Sieme M, Zhazykbayeva S, Herwig M, Budde H, Kovács Á, Kaçmaz M, Göztepe E, et al.(NLRP3) ELISA Kit (ab190807; Abcam), IL-6 ELISA kit (ab100772; Abcam) and 3-nitrotyrosine ELISA kit (ab116691; Abcam), lipid peroxidation (malondialdehyde; LPO) ELISA Kit (ab118970; Abcam), tumor necrosis factor

(11) Diabetes mellitus aggravates myocardial inflammation and oxidative stress in aortic stenosis: a mechanistic link to HFpEF featuresCardiovasc DiabetolMay 13, 2025Melissa Herwig, Marcel Sieme, Andrea Kovács, Muchtiar Khan, Andreas Mügge, Wolfgang E. Schmidt, Ferhat Elci, Shan Sasidharan, Peter Haldenwang, Jan Wintrich, et al.IL-6 ELISA kit (ab100772; Abcam) and IL-18 ELISA kit (ab215539; Abcam), 3-nitrotyrosine ELISA kit (ab116691; Abcam), lipid peroxidation (malondialdehyde; LPO) ELISA Kit (ab118970; Abcam). Total glutathione in

(12) PDE9A Inhibition Improves Coronary Microvascular Rarefaction and Left Ventricular Diastolic Dysfunction in the ZSF1 Rat Model of HFpEFMicrocirculationNovember 1, 2024Katie Anne Fopiano, Saltanat Zhazykbayeva, Ibrahim El‐Battrawy, Vadym Buncha, William M. Pearson, Davis J. Hardell, Liwei Lang, Nazha Hamdani, Zsolt Bagi3-nitrotyrosine was tested with enzyme-linked immunosorbent assay (ELISA) and colorimetric assay kit (ab116691; Abcam). Stable H2O2 accumulation was assessed in myocardial homogenates using a colorimetric assay

(13) Microglial activation induces nitric oxide signalling and alters protein S‐nitrosylation patterns in extracellular vesiclesJournal of Extracellular VesiclesJune 17, 2024Natasha Vassileff, Jereme G. Spiers, Sarah E. Bamford, Rohan G. T. Lowe, Keshava K. Datta, Paul J. Pigram, Andrew F. HillELISA Quantification of 3‐nitrotyrosine levels was achieved using the 3‐NT (3‐Nitrotyrosine) ELISA Kit (Abcam ab116691) as per the manufacturer's instructions. Briefly, cells seeded in 6‐well plates were collected at 1.2

(14) Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathwayJournal of cellular and molecular medicineDecember 1, 2023Chen F, Xiong BR, Xian SY, Zhang J, Ding RW, Xu M, Zhang ZZ.(3‐NT) and 8‐hydroxy 2 deoxyguanosine (8‐OHdG) were detected using commercial kits (#ab118970 for MDA, #ab116691 for 3‐NT, #ab201734 for 8‐OHdG; Abcam) to evaluate oxidative damage of lipids, proteins and nucleic

(15) Endothelial MICU1 alleviates diabetic cardiomyopathy by attenuating nitrative stress-mediated cardiac microvascular injuryCardiovasc DiabetolAugust 17, 2023Xide Shi, Chao Liu, Jiangwei Chen, Shiqiang Zhou, Yajuan Li, Xingcheng Zhao, Jinliang Xing, Junhui Xue, Fengzhou Liu, Fei Li3-nitrotyrosine 3-Nitrotyrosine (3-NT) was determined using the 3-Nitrotyrosine ELISA Kit (Abcam, USA, ab116691) according to the manufacturers’ instructions. CMECs were routinely digested, collected and centrifuged

(16) Tisp40 prevents cardiac ischemia/reperfusion injury through the hexosamine biosynthetic pathway in male miceNature communicationsJune 8, 2023Zhang X, Hu C, Ma ZG, Hu M, Yuan XP, Yuan YP, Wang SS, Kong CY, Teng T, Tang QZ.were purchased from Beyotime (Shanghai, China). LDH assay kit (#ab102526) and 3-NT ELISA assay kit (#ab116691) were obtained from Abcam (Cambridge, UK). THA (#sc-24017), TUN (#sc-3506) and DIT (#sc-29089) were

(17) Hyperbaric oxygenation applied before or after mild or hard stress: effects on the redox state in the muscle tissueThe Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of PharmacologyJanuary 1, 2023Pérez-Castro CC, Kormanovski A, Guevara-Balcázar G, Castillo-Hernández MDC, García-Sánchez JR, Olivares-Corichi IM, López-Sánchez P, Rubio-Gayosso I.homogenates by the enzyme-linked immunosorbent assay (ELISA) with the 3-nitrotyrosine ELISA kit (No. ab116691; Abcam, Cambridge, MA, USA), is expressed as ng/mg of proteins. The level of BH4, also determined by

(18) Comparative treatment with hyperbaric oxygen therapy in a model of systemic loxoscelism in ratsIranian journal of basic medical sciencesDecember 1, 2022Toledo-Blas M, Franco-Vadillo A, Somilleda-Ventura SA, Dominguez-Ruiz B, Guevara-Balcazar G, Kormanovski-Kovzova A, Lopez-Sanchez P, Jarillo-Luna RA, Lara-Padilla E, Castillo-Hernandez MC.device before quantification. The level of 3-nitrotyrosine (3NT, 3-Nitrothyrosine Elisa Kit, Abcam, No. ab116691, UK) was established in homogenates by enzyme-linked immunosorbent assay. SOD and CAT values are expressed

(19) Peroxisome proliferator-activated receptor gamma agonist ELB00824 suppresses oxaliplatin-induced pain, neuronal hypersensitivity, and spinal oxidative stressNeuropharmacologyNovember 1, 2022Morgan Zhang, Min Hu, Sascha R.A. Alles, Marena A. Montera, Ian Adams, Maria D. Santi, Kenji Inoue, Nguyen Huu Tu, Karin N. Westlund, Yi Yein the spinal cord tissue homogenates was determined with the aid of the 3-Nitrotyrosine ELISA kit (Ab116691; Abcam, Cambridge, MA, USA). Protein carbonylated group level was determined with an assay (Colombo

(20) Dimethylarginine dimethylaminohydrolase 1 protects PM2.5 exposure-induced lung injury in mice by repressing inflammation and oxidative stressParticle and Fibre ToxicologyOctober 14, 2022Junling Gao, Tong Lei, Hongyun Wang, Kai Luo, Yuanli Wang, Bingqing Cui, Zhuoran Yu, Xiaoqi Hu, Fang Zhang, Yingjie Chen, et al.ELISA kits for mouse TNFα, IL-6, 3′-NT and 4-HNE were purchased from Abcam PLC (#ab208348, #ab100712, #ab116691 and #ab238538, Cambridge, UK). The Masson’s trichrome staining kit was obtained from Solarbio Science

Be the First to Write a Review!

3-Nitrotyrosine ELISA Kit from Abcam

Abcam

Citations (63)

Reviews

3-Nitrotyrosine ELISA Kit from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map