JC-1 - Mitochondrial Membrane Potential Assay Kit ab113850 from Abcam

Product Specs
ItemJC-1 - Mitochondrial Membrane Potential Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab113850
Quantity100 Test
MethodFuncS
Sample TypeAdherent cells, Suspension cells
Assay Time1h 0m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

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Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Exendin-4 protects brain endothelial cell damage against hyperammonemic conditionBiochem Biophys RepMay 22, 2026Seo Yeon Ahn, Danbi Jo, Seo Yoon Choi, Juhyun SongMitochondrial membrane potential (MMP) was assessed using the JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850, Cambridge, UK) according to the instructions of the manufacturer. Briefly, treated bEnd.3 cells were

(2) ATOX1 alleviates radiation-induced cardiac injury by modulating AMPK/NRF2 to inhibit myocardial oxidative stress and mitochondrial dysfunctionJ Cell Commun SignalMay 15, 2026Wen Deng, Li Sumitochondrial membrane potential, we used the JC‐1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850, UK). Cells were seeded in a 96‐well plate at a density of 1.5 × 104 cells per well and cultured overnight.

(3) Baicalein Protects H9c2 Cardiomyoblasts Against LPS-Induced Inflammatory Injury by Modulating the NF-κB/NLRP3 Inflammasome Pathway and Mitochondrial ROSIran J Pharm ResApril 28, 2026Yi Lu, Zahra Zahid Piracha, Umar Saeed, Fangjun LuoLDH Cytotoxicity Detection Kit: Takara Bio, Cat. No. MK401 - JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam, Cat. No. ab113850 - DCFH-DA ROS Detection Probe: Sigma-Aldrich, Cat. No. D6883 - TRIzol™ Reagent: Invitrogen, Cat. No.

(4) Incense Aerosol-Induced Neurotoxicity Disrupts α-Synuclein Homeostasis in a Cellular Parkinson’s Disease Model, Distinct from Cigarette AerosolsChemical Research in ToxicologyApril 20, 2026Yi-En Tseng, Ming-Chu Teng, Yu-Siou Huang, Chia-Hsuan Pan, Yuan-Pin Chang, Chia C. Wang, Hsiu-Fang FanMitochondrial Membrane Potential Assay Mitochondrial membrane potential was assessed using JC-1 (Abcam, ab113850) following the manufacturer’s instructions. Carbonyl cyanide m-chlorophenyl hydrazone (CCCP) disrupts

(5) Differential Effects of Phenanthrene and Its Chlorinated Congeners on Hormone Production and Mitochondrial Function in Ovarian Granulosa CellsToxicsApril 7, 2026Genevieve A. Perono, Laiba Jamshed, Rohita Dutt, Reese S. Cameron, James J. Petrik, Philippe J. Thomas, Alison C. Hollowaychlorinated Phe was evaluated using the fluorescent JC-1 Mitochondrial Membrane Potential Assay Kit (ab113850, Abcam), according to the manufacturer’s protocol (N = 5–8 independent experiments). Cells were seeded

(6) Bioactivity-Guided Fractionation of the Bidah Pomegranate Landrace Identifies a Bioactive Fraction Inducing Mitochondria-Associated Apoptotic Responses in Colorectal Cancer CellsInt J Mol SciMarch 20, 2026Saheed O. Anifowose, Nada M. Alattas, Khalid M. Al-Rohily, Abdalrhaman M. SalihAssay Mitochondrial membrane potential (ΔΨm) was evaluated using the Abcam JC-1 fluorescent probe (ab113850, Abcam plc, Cambridge, UK). Caco-2 cells were incubated with the B6 fraction at 50 µg/mL for 24 h. FCCP

(7) Size-Segregated Incense Aerosols Drive ROS–Mitochondrial Dysfunction and Programmed Cell Death Across Human Cell TypesChemical Research in ToxicologyMarch 16, 2026Yi-En Tseng, Ming-Chu Teng, Yu-Siou Huang, Padhmavathi Selvam, Chia-Hsuan Pan, Yuan-Pin Chang, Chia C. Wang, Hsiu-Fang Fan(α-plus, 116,196–3SB). For measurement of MMP, cells were preincubated with 100 μL of JC-1 (Abcam, ab113850) for 10 min. Then cells were washed with 1X dilution buffer twice before treatment with IAE obtained

(8) Prophylactic C-terminal occludin-derived peptide attenuates LPS-induced airway inflammation via barrier preservation and mitochondrial ROS regulationRedox BiolMarch 10, 2026Hyun-Chae Lee, Jeongtae Kim, Hye Min Jeong, Jaewon Shim, Tae-Jin Lee, Sangwook Lim, Suk Won Chang, Kyoung Seob Songenergized mitochondria. The assay was performed using the JC-1 Mitochondrial Membrane Potential Assay Kit (ab113850, Abcam, Cambridge, UK) according to the manufacturer's instructions. 2.12 Animal experiments All animal experiments

(9) Adiponectin Inhibits Oxidative Stress and Tight Junction Protein Loss: Evidence from a Hepatic Encephalopathy Mouse Model and Brain Endothelial CellsPharmaceuticals (Basel)March 4, 2026Dong Jun Song, Seol Won Jeong, Seoyeon Ahn, Danbi Jo, Che-Hun Jung, Jiwoun Park, Sangjun Lee, Juhyun SongJC-1 mitochondrial membrane potential assay kit (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide; ab113850, Abcam, Cambridge, UK). For fluorescence imaging, cells were seeded in 24-well plates at 6 × 104 cells/well.

(10) Microplastic-Induced Macrophage Dysfunction Drives Lung Tumor Progression through Glutathione ImbalanceACS NanoFebruary 24, 2026Bora Kim, Koung-Min Park, Haerang Lee, Young-Min HyunCO2, with or without polystyrene MPs, and then harvested. Samples were stained with JC-1 dye (Abcam, ab113850) at a final concentration of 5 μM for 20 min at 37 °C in an incubator. Samples were measured via flow cytometry analysis

(11) 3D Bioprinted Fat‐Myocardium Model Unravels the Role of Adipocyte Hypertrophy in Atrial DysfunctionAdvanced ScienceFebruary 8, 2026Lara Ece Celebi, Pinar Zorlutunawhereas the 3D OAdi group exhibited low adiponectin and high leptin levels (Figure S1F‐G). JC‐1 (Abcam, ab113850) was conducted as reported before [157], and CellROX (Thermo Fisher Scientific, C10444) assay were conducted

(12) Cannabis smoke extract disrupts trophoblast differentiation and causes mitochondrial dysfunction beyond the effects of Δ9-THC aloneScientific ReportsJanuary 26, 2026Cristina Monaco, Mahek Minhas, Tina Podinic, Joshua P. Nederveen, Amica-Mariae Lucas, Thane Tomy, Gregg T. Tomy, Alison C. Holloway, Sandeep Rahatetraethylbenzimidazolylcarbocyanine iodide (JC-1) with the JC‐1‐Mitochondrial Membrane Potential Assay kit (Abcam, ab113850) following the manufacturer’s instructions. Briefly, cells were seeded in a black, clear bottom 96-well

(13) Estrogen-Induced Hypermethylation Silencing of RPS2 and TMEM177 Inhibits Energy Metabolism and Reduces the Survival of CRC CellsCellsJanuary 9, 2026Batoul Abi Zamer, Bilal Rah, Wafaa Abumustafa, Zheng-Guo Cui, Mawieh Hamad, Jibran Sualeh MuhammadScientific). The MMP was measured using the JC-1-Mitochondrial Membrane Potential Assay kit (Abcam, Cat. No. ab113850). Briefly, following treatment, cells were washed once with 1X dilution buffer and then incubated with

(14) AUF1 Restrains Hepatocyte Senescence by Maintaining Mitochondrial Homeostasis in AML12 Hepatocyte ModelCellsDecember 26, 2025Myeongwoo Jung, Sukyoung Han, Seungyeon Ryu, Seongho Cha, Ye Eun Sim, Se Hoon Jung, Hyosun Tak, Wook Kim, Eun Kyung LeeMitochondrial ToxGlo™ Assay (Promega Corporation; G8000) and the JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam Plc.; ab113850), respectively, according to the manufacturer’s instructions. Luminescence signals from ToxGlo™ Assay

(15) SYK regulates Schwann cell metabolic reprogramming to promote axonal regeneration in immune-mediated neuropathyJournal of NeuroinflammationDecember 6, 2025Nannan Zhang, Chen Liu, Fangyu Chen, Qian Zheng, Yuzhong Wang, Fangzhen Shanmicroscope (Zeiss, Jena, Germany). MMP measurement SCs were incubated with 2 µM JC-1 (Abcam, Cat#ab113850) for 20 min at 37 °C in the dark. After washing, fluorescence (aggregates: Ex/Em 560/595 nm; monomers:

(16) Microplastic Toxicity on Gut Microbiota and Intestinal Cells: Evidence from the Simulator of the Human Intestinal Microbial Ecosystem (SHIME)ToxicsDecember 2, 2025Xingchao Ren, Chen Su, Yuyan Zhu, James Kar-Hei Fang, Pei Yee Wohobtained cells were washed twice with PBS, following treatment with a 10 μM solution of JC-1 dye (Abcam, ab113850). This treatment was carried out at 37 °C for a duration of thirty minutes. Then, the cells were analyzed

(17) Antiretroviral Drugs Impact Autophagy Differently in Primary Human AstrocytesCellsDecember 1, 2025Laura Cheney, Grace McDermott, Hillary Guzik, Joan W. Bermantechnical control. In the last 30 min of treatment, tetraethylbenzimidazolylcarbocyanine iodide (JC-1; #ab113850 [Abcam]) was added for a final concentration of 10 µM. At treatment end, cells were washed with 1x assay

(18) Mitochondrial metabolic rewiring sensitizes mTORC1 inhibitor persister cells to cuproptosisJCI InsightNovember 24, 2025Heng Du, Heng-Jia Liu, Magdalena Losko, Yu Chi Yang, Min Yuan, Elizabeth P. Henske, John M. Asara, Mallika Singh, David J. KwiatkowskiMitochondria Analyzer. Mitochondria membrane potential was measured by JC-1 staining (10 μM, Abcam, ab113850) according to the manufacturer’s recommendations. Mitochondria Analyzer (57) was used for quantification

(19) Cytochrome c Oxidase Subunit 5A (COX5A) Enhances Gastric Cancer Progression by Augmenting ATP Synthesis and Activating the PI3K /Akt PathwayJournal of Cellular and Molecular MedicineNovember 3, 2025Dongyan Li, Limin ZhouAntibodies Luminescent ATP Detection Assay (ab113849), JC1 Mitochondrial Membrane Potential Assay Kit (ab113850), and Anti‐COX5A antibody (ab181226, 1:1000) were purchased from Abcam (Berlin, Germany). Phospho‐PI3K

(20) Knockdown of mitochondrial sirtuin sir-2.2 reduces alpha-synuclein clearance and impairs energy homeostasis in a model of ageingDisease Models & MechanismsNovember 1, 2025Anam Naseer, Pranoy Toppo, Mahmood Akbar, Aamir Nazirre-suspended in serum-free RPMI (Gibco-Thermo Fisher Scientific). For staining, JC-1 dye (2.5 μgml−1; Abcam; ab113850) was used and incubated for 15 min at room temperature. MMP analysis was performed using a flow cytometer

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JC-1 - Mitochondrial Membrane Potential Assay Kit from Abcam

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