Published customer image: LYNX Rapid APC Antibody Conjugation Kit (LNK033) used to conjugate the anti Duck antibody, clone 4A12 to allophycocyanin prior to flow cytometric analysis of Mallard peripheral blood cells.Image caption:Gating strategy. EDTA-blood was stained with 4A12-APC, anti-chBAFF-R (2C4)-PE, anti-duCD4-FITC, anti-duCD8-PerCp, and anti-2G3-APC-Cy7 in a no-lyse no-wash single-step one-tube procedure and analyzed by flow cytometry. Shown is the gating strategy for the separation of thrombocytes and leukocytes (A), followed by doublet exclusion. (B) Leukocytes were classified as CD4 or CD8 single positive or CD4/CD8 double negative cells. (C) By means of their scatter properties, CD4/CD8 double negative cells were subdivided into granulocytes and monocytes. (D) CD8+ leukocytes were classified into 2C4-negative T cells and 2C4-positive B cells. Initial thrombocyte and leukocyte discrimination (A) was followed by doublet exclusion. Fluorescence-based gates were always followed by FSC/SSC gating. FSC/SSC gates were used to sort purify the respective cell populations (purity, >95%) and prepare cytospin slides followed by DiffQuick staining for thrombocytes (I), CD4+ T cells (II), granulocytes (III), monocytes (IV), CD8+ T cells (V), and B cells (VI).From: Jax E, Werner E, Müller I, Schaerer B, Kohn M, Olofsson J, Waldenström J, Kraus RHS, Härtle S.Evaluating Effects of AIV Infection Status on Duc
LYNX RAPID APC ANTIBODY CONJUGATION KIT
Published customer image: LYNX Rapid APC Antibody Conjugation Kit (LNK032APC) used for the conjugation of anti VAP-1 antibody to allophycocyanin for subsequent detection of VAP-1 in colon tissues by immunofluorescence.Image caption:Dual-colour immunofluorescent staining of sections of normal colon tissue and cultured colonic endothelial cells. Magnification x630. a VAP-1 staining is present in SMA+ cells of vessels in sections of colonic mucosa. b VAP-1 staining does not co-localize with CD31+ endothelial staining of vessels in sections of colonic mucosa. Right-most images are digitally magnified x3. Yellow staining represents autofluorescence from erythrocytes. c Positive CD31 staining (left) and absent VAP-1 staining (right) of cultured colonic endothelial cellsFrom: Ward ST, Weston CJ, Shepherd EL, Hejmadi R, Ismail T, Adams DH.Evaluation of serum and tissue levels of VAP-1 in colorectal cancer..
Published customer image: LYNX Rapid APC Antibody Conjugation Kit (LNK032APC) used for the conjugation of anti VAP-1 antibody to allophycocyanin for subsequent detection of VAP-1 in colon tissues by immunofluorescence.Image caption:Dual-colour immunofluorescent staining of sections of normal colon tissue and cultured colonic endothelial cells. Magnification x630. a VAP-1 staining is present in SMA+ cells of vessels in sections of colonic mucosa. b VAP-1 staining does not co-localize with CD31+ endothelial staining of vessels in sections of colonic mucosa. Right-most images are digitally magnified x3. Yellow staining represents autofluorescence from erythrocytes. c Positive CD31 staining (left) and absent VAP-1 staining (right) of cultured colonic endothelial cellsFrom: Ward ST, Weston CJ, Shepherd EL, Hejmadi R, Ismail T, Adams DH.Evaluation of serum and tissue levels of VAP-1 in colorectal cancer.BMC Cancer. 2016 Feb 24;16(1):154.
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