Fig 1: Simplified coagulation cascade and complement system featuring transcriptomics, proteomics, and metabolomics regulations of systemic lupus erythematosus patients compared with healthy controls, according to KEGG nomenclatureGenes are presented as ovals, proteins as rectangles, metabolites by trapezoid. Regulation is color coded in which red stands for up regulated, green for down regulated, and black for unregulated. TFPI, tissue factor pathway inhibitor; F3, coagulation factor 3; F7, coagulation factor 7; F8, coagulation factor 8; F9, coagulation factor 9; F11, coagulation factor 11; F12, coagulation factor 12; F13, coagulation factor 13; F2, coagulation factor 2; F2R, F2 receptor; VWF, Von Willebrand factor; ATIII, antithrombin-III; PROZ, protein Z; PROS, protein S; FIB, fibrinogen; IF, complement factor I; DF, complement factor D; MAC, membrane attack complex; MASP2, mannan-binding lectin-associated serine protease 2; C4BP, C4b-binding protein; KEGG, kyoto encyclopedia of genes and genomes.
Fig 2: SERPINC1 (antithrombin gene) expression and quantification of AT and TAT in ECs and fibroblasts. (a) The expression levels of SERPINC1 in each cell type were analyzed by real time-PCR and quantified relative to HUVEC levels after normalization to GAPDH in each PCR analysis. Data shown are means + SD from three separate RNA extractions for each cell type. *p < 0.0001. (b) Levels of AT via ELISA were quantified in supernatant of HUVECs (n = 3), LSECs (n = 4), GMVECs (n = 3), and fibroblasts (n = 5). (c) Quantification of TAT in the lysate/cell membrane fraction of HUVECs, LSECs, GMVECs, and fibroblasts (n = 3 for each cell type). *p < 0.05.
Supplier Page from Abcam for Human Thrombin-Antithrombin Complex ELISA Kit (TAT)