Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to malonyl CoA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated malonyl CoA. The competitive inhibition reaction is launched between with HRP-conjugated malonyl CoA and malonyl CoA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of malonyl CoA in the sample. The color development is stopped and the intensity of the color is measured