Lysyl Oxidase Activity Assay Kit (Fluorometric) ab112139 from Abcam

Product Specs
ItemLysyl Oxidase Activity Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112139
MethodFuncS
Quantity500 Test
Sample TypeCell culture supernatant, Cell culture extracts, Tissue Extracts
SensitivityEqual to 40 ng/well
Assay Time0h 30m
ApplicationsEnzyme activity assay
Research AreaImmuno-oncology
NCBI Full Gene Namelysyl oxidase like 1
NCBI Gene AliasesLOL, LOXL

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Fatty Acid Oxidation‐Glycolysis Metabolic Transition Affects ECM Homeostasis in Silica‐Induced Pulmonary FibrosisAdvanced ScienceDecember 25, 2024Wenqing Sun, Siyun Zhou, Lan Peng, Wei Wang, Yi Liu, Ting Wang, Demin Cheng, Ziwei Li, Haojie Xiong, Xinying Jia, et al.activity in cells and culture medium was determined by LOX Activity Assay Kit (Fluorometric, Abcam, ab112139). According to the manufacturer's plan, the activity of LOX enzyme is detected using a red fluorescence

(2) A highly potent bi-thiazole inhibitor of LOX rewires collagen architecture and enhances chemoresponse in triple-negative breast cancerCell chemical biologyNovember 21, 2024Cetin M, Saatci O, Rezaeian AH, Rao CN, Beneker C, Sreenivas K, Taylor H, Pederson B, Chatzistamou I, Buckley B, et al.assays,Critical commercial assays,Critical commercial assays Lysyl Oxidase Activity Assay (Cell-based),Abcam,Cat#ab112139 Lysyl Oxidase Activity Assay (Recombinant protein-based),Abcam,Cat#ab284573 Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit

(3) Gene inactivation of lysyl oxidase in smooth muscle cells reduces atherosclerosis burden and plaque calcification in hyperlipidemic miceAtherosclerosisOctober 1, 2024Filipe F. Stoyell-Conti, Maya Suresh Kumar, Zachary M. Zigmond, Miguel G. Rojas, Nieves Santos Santos Falcon, Laisel Martinez, Roberto I. Vazquez-Padronsolution. For the LOX activity in the aorta, samples were tested using LOX activity assay kit (Abcam, ab112139). The assay uses a LOX substrate that releases hydrogen peroxide upon transformation by the LOX present

(4) Intermittent cyclic stretch of engineered ligaments drives hierarchical collagen fiber maturation in a dose- and organizational-dependent mannerActa BiomaterialiaSeptember 1, 2024Leia D. Troop, Jennifer L. Puetzerprotease inhibitor at each time point and frozen at −80 °C. A fluorometric LOX activity assay (Abcam, ab112139) was used to determine arbitrary units (A.U.) of LOX activity. The assay was run according to manufacturer

(5) LOX, but not LOXL2, promotes bone metastasis formation and bone destruction in triple-negative breast cancerJournal of Bone OncologyFebruary 1, 2024Paola Di Mauro, Martine Croset, Lamia Bouazza, Philippe Clézardin, Caroline Reynaud°C. 3 Lysyl oxidase activity assay The assay was performed according to the manufacturer instructions (ab112139 Abcam). Briefly, 50 μL of cell conditioned media, or increasing concentrations of recombinant LOX (Creative

(6) Dysregulation of extracellular matrix and Lysyl Oxidase in Ehlers-Danlos syndrome type IV skin fibroblastsOrphanet journal of rare diseasesJanuary 5, 2024Foehr R, Anderson K, Dombrowski O, Foehr A, Foehr ED.Cat#MBS450320). Lysyl oxidase activity assay Samples were tested using LOX activity assay kit (Abcam, ab112139) to measure lysyl oxidase activity. The assay uses a LOX substrate that releases hydrogen peroxide upon

(7) S100A4 Is a Key Facilitator of Thoracic Aortic DissectionInternational Journal of Biological SciencesJanuary 1, 2024Jiajun Shi, Wenjun Yu, Chuan Liang, Hongjie Shi, Dengwei Cao, Yong Ran, Haisen Qiao, Zhe Dong, Jinping Liurelative activity assay A semi-quantitative activity assay was performed using a LOX Activity Assay Kit (ab112139, Abcam). First, we collected the VSMCs culture supernatant and centrifuged the samples. After centrifugation,

(8) Mesenchymal Stromal Cell Secretome Is Affected by Tissue Source and Donor AgeStem CellsNovember 5, 2023Agnieszka J Turlo, Dean E Hammond, Kerry A Ramsbottom, Jamie Soul, Alexandra Gillen, Kieran McDonald, Mandy J Pefferslysyl oxidase (LOX) in CM samples (n = 27) was measured using fluorometric commercial assay (Abcam, ab112139) following the manufacturer’s protocol. The relationship between LOX activity and donor age was evaluated

(9) Temporal application of lysyl oxidase during hierarchical collagen fiber formation differentially effects tissue mechanicsActa BiomaterialiaApril 1, 2023Madison E. Bates, Leia Troop, M. Ethan Brown, Jennifer L. Puetzerweeks. Media was frozen, stored at −80°C, and analyzed with a fluorometric LOX activity assay (Abcam, ab112139). The assay was performed according to manufactures protocol with media samples taken throughout culture

(10) Critical involvement of lysyl oxidase in seizure-induced neuronal damage through ERK-Alox5-dependent ferroptosis and its therapeutic implicationsActa Pharmaceutica Sinica BSeptember 1, 2022Xiaoyuan Mao, Xuan Wang, Mingzhu Jin, Qin Li, Jining Jia, Menghuan Li, Honghao Zhou, Zhaoqian Liu, Weilin Jin, Yanli Zhao, et al.analyzed at 450 nm. 2.15 Detection of LysOX activity LysOX activity was detected by an assay kit (ab112139, Abcam, UK) based on the standard protocol. 2.16 Gene knockdown The knockdown of selected genes was

(11) Glioblastoma‐educated mesenchymal stem‐like cells promote glioblastoma infiltration via extracellular matrix remodelling in the tumour microenvironmentClinical and Translational MedicineAugust 1, 2022Seung‐Mo Kim, Eun‐Jung Lim, Ki‐Chun Yoo, Yi Zhao, Jae‐Hyeok Kang, Eun‐Ji Lim, Incheol Shin, Seok‐Gu Kang, Han Woong Lim, Su‐Jae Leemanufacturer's instructions. The activity of LOX protein was measured using a Lysyl Oxidase Activity Assay kit (ab112139, Abcam, Cambridge, UK) in recombinant active LOX protein or collected CM from each sample according

(12) IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusionThe Journal of experimental medicineJune 6, 2022Chen X, Zhao J, Herjan T, Hong L, Liao Y, Liu C, Vasu K, Wang H, Thompson A, Fox PL, et al.activity assay and hydroxyproline assay For all LOX activity assays (Lysyl oxidase Activity Assay Kit, ab112139; Abcam), products in the last step were incubated at 37°C extendedly up to 3 h and then measured at

(13) A New Generation of Ultrasmall Nanoparticles Inducing Sensitization to Irradiation and Copper Depletion to Overcome Radioresistant and Invasive CancersPharmaceuticsApril 7, 2022Paul Rocchi, Delphine Brichart-Vernos, François Lux, Isabelle Morfin, Laurent David, Claire Rodriguez-Lafrasse, Olivier Tillementcondition was collected and LOX activity was determined using LOX Activity Fluorometric Assay Kit (ab112139, Abcam, Cambridge, UK) according to the manufacturer’s instructions. Briefly, the cell culture medium

(14) Sphingosine kinase 1 regulates lysyl oxidase through STAT3 in hyperoxia-mediated neonatal lung injuryThoraxJanuary 1, 2022Ha AW, Bai T, Ebenezer DL, Sethi T, Sudhadevi T, Mangio LA, Garzon S, Pryhuber GS, Natarajan V, Harijith A.prior to stimulation with HO (95% O2, 5%CO2) for 6 hours. LOX activity assay Kit (Fluorometric) (Ab112139, Abcam) was used to measure LOX activity in BAL and cell culture supernatant solutions. The assay uses

(15) CCR5 signaling promotes lipopolysaccharide-induced macrophage recruitment and alveolar developmental arrestCell death & diseaseFebruary 15, 2021Chen Z, Xie X, Jiang N, Li J, Shen L, Zhang Y.activation of LOX in lung tissues was assessed using a fluorometric LOX activity assay kit (Cat. # ab112139; Abcam) according to the manufacturer’s instructions. Flow cytometry Peripheral blood mononuclear

(16) Dopamine D1 receptor stimulates cathepsin K-dependent degradation and resorption of collagen I in lung fibroblastsJournal of cell scienceDecember 11, 2020Diaz-Espinosa AM, Link PA, Sicard D, Jorba I, Tschumperlin DJ, Haak AJ.each condition was collected, and LOX activity was determined using the fluorometric LOX activity assay kit (ab112139, Abcam, Cambridge, UK), as per the manufacturer’s instructions. Briefly, cell culture supernatant was collected

(17) Longitudinal Imaging Using PET/CT with Collagen-I PET-Tracer and MRI for Assessment of Fibrotic and Inflammatory Lesions in a Rat Lung Injury ModelJournal of Clinical MedicineNovember 18, 2020Irma Mahmutovic Persson, Nina Fransén Pettersson, Jian Liu, Hanna Falk Håkansson, Anders Örbom, René In ’t Zandt, Ritha Gidlöf, Marie Sydoff, Karin von Wachenfeldt, Lars E. Olssonmeasured in the right inferior lung lobe using the fluorometric Lysyl Oxidase Activity Assay Kit (Abcam, ab112139) according to manufacturer’s protocol. In brief, 1 mg/mL total protein from homogenized lung tissue

(18) Lysyl Oxidase Inhibition Ablates Sexual Dimorphism of Abdominal Aortic Aneurysm Formation in MiceCirculationNovember 17, 2020Michihiro Okuyama et al.mice infused with either saline or AngII (1000 ng·kg–1·min–1 for 5 days) than in female mice (Abcam, ab112139; n=4, *P<0.05 female vs male; 2-way ANOVA followed by Holm-Sidak post hoc test). D, Orchiectomy (ORCH)

(19) Metallothioneins regulate ATP7A trafficking and control cell viability during copper deficiency and excessScientific reportsMay 12, 2020Gudekar N, Shanbhag V, Wang Y, Ralle M, Weisman GA, Petris MJ.cells after 48 h culture were used to measure lysyl oxidase activity using a LOX activity kit (Abcam, ab112139), according to the manufacturer’s instructions. Metal measurements were performed using inductively

(20) Tumor-Stroma Mechanics Coordinate Amino Acid Availability to Sustain Tumor Growth and MalignancyCell metabolismJanuary 8, 2019Bertero T, Oldham WM, Grasset EM, Bourget I, Boulter E, Pisano S, Hofman P, Bellvert F, Meneguzzi G, Bulavin DV, et al.450nm. LOX activity assay Lox activity was measured using the Lysyl Oxidase Activity Assay Kit (Abcam; Ab112139), following the manufacturer instructions and as we previously described. Briefly, 5μg of total protein

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Lysyl Oxidase Activity Assay Kit (Fluorometric) from Abcam

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Lysyl Oxidase Activity Assay Kit (Fluorometric) from Abcam

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